Biology and Manipulation Technologies of Male Germline Stem Cells in Mammals

Overview

Journal Reprod Med Biol

Specialty Biology

Date 2018 Nov 1

PMID 30377393

Citations 5

Authors

Seiji Takashima

Affiliations

Soon will be listed here.

Abstract

Background: Spermatogonial stem cells (SSCs) are the origin of sperm and defined by their functions of "colonization in the testis" and "spermatogenesis". In vitro manipulation techniques of SSCs contribute to a wide variety of fields including reproductive medicine and molecular breeding. This review presents the recent progress of the biology and manipulation technologies of SSCs.

Methods: Research articles regarding SSC biology and technologies were collected and summarized.

Main Findings: Dr. Ralph Brinster developed the spermatogonial transplantation technique that enables SSC detection by functional markers. Using this technique, cultured SSCs, termed germline stem (GS) cells, were established from the mouse. GS cells provide the opportunity to produce genome-edited animals without using zygotes. In vitro spermatogenesis allows production of haploid germ cells from GS cells without spermatogonial transplantation. The recent advancement of pluripotent stem cell culture techniques has also achieved production of functional GS-like cells in addition to male/female germ cells.

Conclusion: Although in vitro manipulation techniques of GS cells have been developed for the mouse, it appears to be difficult to apply these techniques to other species. Understanding and control of interspecies barriers are required to extend this technology to nonrodent mammals.

Citing Articles

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Lipofection of Non-integrative CRISPR/Cas9 Ribonucleoproteins in Male Germline Stem Cells: A Simple and Effective Knockout Tool for Germline Genome Engineering.

Obermeier M, Vadolas J, Verhulst S, Goossens E, Baert Y Front Cell Dev Biol. 2022; 10:891173.

PMID: 35774227 PMC: 9237505. DOI: 10.3389/fcell.2022.891173.

and Determinations of The Anti-GDNF Family Receptor Alpha 1 Antibody in Mice by Immunochemistry and RT-PCR.

Azizi H, Tabar A, Skutella T, Govahi M Int J Fertil Steril. 2020; 14(3):228-233.

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Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency.

Tsuchimoto A, Tone M, Ogonuki N, Hada M, Ogura A, Takashima S Sci Rep. 2020; 10(1):10796.

PMID: 32612133 PMC: 7330030. DOI: 10.1038/s41598-020-67595-1.

Biology and manipulation technologies of male germline stem cells in mammals.

Takashima S Reprod Med Biol. 2018; 17(4):398-406.

PMID: 30377393 PMC: 6194257. DOI: 10.1002/rmb2.12220.

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