Restriction of P-element Insertions at the Notch Locus of Drosophila Melanogaster

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1987 Apr 1

PMID 3037327

Citations 30

Authors

M R Kelley

S Kidd

R L Berg

M W YOUNG

Affiliations

Soon will be listed here.

Abstract

P elements move about the Drosophila melanogaster genome in a nonrandom fashion, preferring some chromosomal targets for insertion over others (J. C. J. Eeken, F. H. Sobels, V. Hyland, and A. P. Schalet, Mutat. Res. 150:261-275, 1985; W. R. Engels, Annu. Rev. Genet. 17:315-344, 1983; M. D. Golubovsky, Y. N. Ivanov, and M. M. Green, Proc. Natl. Acad. Sci. USA 74:2973-2975, 1977; M. J. Simmons and J. K. Lim, Proc. Natl. Acad. Sci. USA 77:6042-6046, 1980). Some of this specificity may be due to recognition of a particular DNA sequence in the target DNA; derivatives of an 8-base-pair consensus sequence are occupied by these transposable elements at many different chromosomal locations (K. O'Hare and G. M. Rubin, Cell 34:25-36, 1983). An additional level of specificity of P-element insertions is described in this paper. Of 14 mutations induced in the complex locus Notch by hybrid dysgenesis, 13 involved P-element insertions at or near the transcription start site of the gene. This clustering was not seen in other transposable element-induced mutations of Notch. DNA sequences homologous to the previously described consensus target for P-element insertion are not preferentially located in this region of the locus. The choice of a chromosomal site for integration appears to be based on more subtle variations in chromosome structure that are probably associated with activation or expression of the target gene.

Citing Articles

Expression of a reporter gene resembles that of its neighbour: an insertion in the hairy gene of Drosophila.

Fasano L, Core N, Kerridge S Rouxs Arch Dev Biol. 2017; 197(8):507-512.

PMID: 28305477 DOI: 10.1007/BF00385685.

Raissa L. Berg's contributions to the study of phenotypic integration, with a professional biographical sketch.

Conner J, Lande R Philos Trans R Soc Lond B Biol Sci. 2014; 369(1649):20130250.

PMID: 25002696 PMC: 4084536. DOI: 10.1098/rstb.2013.0250.

Hsmar1 transposition is sensitive to the topology of the transposon donor and the target.

Claeys Bouuaert C, Chalmers R PLoS One. 2013; 8(1):e53690.

PMID: 23341977 PMC: 3544897. DOI: 10.1371/journal.pone.0053690.

Interactions between enhancer of rudimentary and Notch and deltex reveal a regulatory function of enhancer of rudimentary in the Notch signaling pathway in Drosophila melanogaster.

Tsubota S, Vogel A, Phillips A, Ibach S, Weber N, Kostrzebski M Fly (Austin). 2011; 5(4):275-84.

PMID: 21904119 PMC: 3266069. DOI: 10.4161/fly.5.4.17807.

Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila.

Witsell A, Kane D, Rubin S, McVey M Genetics. 2009; 183(3):1187-93.

PMID: 19687136 PMC: 2778970. DOI: 10.1534/genetics.109.108472.

References

Snyder M, Kimbrell D, Hunkapiller M, Hill R, Fristrom J, Davidson N . A transposable element that splits the promoter region inactivates a Drosophila cuticle protein gene. Proc Natl Acad Sci U S A. 1982; 79(23):7430-4. PMC: 347353. DOI: 10.1073/pnas.79.23.7430. View

Kidd S, Lockett T, YOUNG M . The Notch locus of Drosophila melanogaster. Cell. 1983; 34(2):421-33. DOI: 10.1016/0092-8674(83)90376-8. View

Tsubota S, Ashburner M, Schedl P . P-element-induced control mutations at the r gene of Drosophila melanogaster. Mol Cell Biol. 1985; 5(10):2567-74. PMC: 366991. DOI: 10.1128/mcb.5.10.2567-2574.1985. View

Engels W, Preston C . Hybrid dysgenesis in Drosophila melanogaster: the biology of female and male sterility. Genetics. 1979; 92(1):161-74. PMC: 1213938. DOI: 10.1093/genetics/92.1.161. View

Yedvobnick B, Muskavitch M, Wharton K, Halpern M, Paul E, Grimwade B . Molecular genetics of Drosophila neurogenesis. Cold Spring Harb Symp Quant Biol. 1985; 50:841-54. DOI: 10.1101/sqb.1985.050.01.102. View

Kidwell M, Kidwell J, Sved J . Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977; 86(4):813-33. PMC: 1213713. DOI: 10.1093/genetics/86.4.813. View

Eeken J, SOBELS F, Hyland V, Schalet A . Distribution of MR-induced sex-linked recessive lethal mutations in Drosophila melanogaster. Mutat Res. 1985; 150(1-2):261-75. DOI: 10.1016/0027-5107(85)90122-8. View

Kidd S, Kelley M, YOUNG M . Sequence of the notch locus of Drosophila melanogaster: relationship of the encoded protein to mammalian clotting and growth factors. Mol Cell Biol. 1986; 6(9):3094-108. PMC: 367044. DOI: 10.1128/mcb.6.9.3094-3108.1986. View

Welshons W, Welshons H . Suppression of the facet-strawberry position effect in Drosophila by lesions adjacent to notch. Genetics. 1985; 110(3):465-77. PMC: 1202575. DOI: 10.1093/genetics/110.3.465. View

10.

Engels W . The P family of transposable elements in Drosophila. Annu Rev Genet. 1983; 17:315-44. DOI: 10.1146/annurev.ge.17.120183.001531. View

11.

Simmons M, Lim J . Site specificity of mutations arising in dysgenic hybrids of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980; 77(10):6042-6. PMC: 350209. DOI: 10.1073/pnas.77.10.6042. View

12.

Green M . Genetic instability in Drosophila melanogaster: De novo induction of putative insertion mutations. Proc Natl Acad Sci U S A. 1977; 74(8):3490-3. PMC: 431616. DOI: 10.1073/pnas.74.8.3490. View

13.

Wharton K, Johansen K, Xu T, Artavanis-Tsakonas S . Nucleotide sequence from the neurogenic locus notch implies a gene product that shares homology with proteins containing EGF-like repeats. Cell. 1985; 43(3 Pt 2):567-81. DOI: 10.1016/0092-8674(85)90229-6. View

14.

McGinnis W, Shermoen A, Beckendorf S . A transposable element inserted just 5' to a Drosophila glue protein gene alters gene expression and chromatin structure. Cell. 1983; 34(1):75-84. DOI: 10.1016/0092-8674(83)90137-x. View

15.

OHare K, Rubin G . Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983; 34(1):25-35. DOI: 10.1016/0092-8674(83)90133-2. View

16.

Searles L, Greenleaf A, KEMP W, Voelker R . Sites of P element insertion and structures of P element deletions in the 5' region of Drosophila melanogaster RpII215. Mol Cell Biol. 1986; 6(10):3312-9. PMC: 367076. DOI: 10.1128/mcb.6.10.3312-3319.1986. View

17.

Biggin M, Gibson T, Hong G . Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983; 80(13):3963-5. PMC: 394179. DOI: 10.1073/pnas.80.13.3963. View

18.

Artavanis-Tsakonas S, Muskavitch M, Yedvobnick B . Molecular cloning of Notch, a locus affecting neurogenesis in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983; 80(7):1977-81. PMC: 393735. DOI: 10.1073/pnas.80.7.1977. View

19.

Golubovsky M, Ivano Y, Green M . Genetic instability in Drosophila melanogaster: putative multiple insertion mutants at the singed bristle locus. Proc Natl Acad Sci U S A. 1977; 74(7):2973-5. PMC: 431368. DOI: 10.1073/pnas.74.7.2973. View

20.

Ikenaga H, Saigo K . Insertion of a movable genetic element, 297, into the T-A-T-A box for the H3 histone gene in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982; 79(13):4143-7. PMC: 346593. DOI: 10.1073/pnas.79.13.4143. View