P-element-induced Control Mutations at the R Gene of Drosophila Melanogaster

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1985 Oct 1

PMID 3016507

Citations 30

Authors

S Tsubota

M Ashburner

P Schedl

Affiliations

Soon will be listed here.

Abstract

The P-M hybrid dysgenesis system was used to produce five putative regulatory mutations at the rudimentary locus, r. All five mutations were the result of insertions at the 5' end of the gene, upstream of the proposed start of transcription. All of the mutants displayed a leaky wing phenotype, and four of the mutants showed an uncoupling of the wing and female-sterility phenotypes, suggesting that they altered the normal spatial and temporal expression of the r gene. Four of the insertions were P elements. The fifth insertion, which was larger than an intact P element, consisted of a small P element connected to non-P-element DNA. Two of the mutants produced very little r transcript in adult females and were clustered 80 to 150 base pairs upstream of the start of transcription. The other three mutants had higher levels of r transcript in adult females and were clustered 440 to 500 base pairs upstream of the start of transcription. All of the data suggest that the insertions are in a 5' noncoding region of the r gene involved in the control of its spatial and temporal expression.

Citing Articles

Expression of a reporter gene resembles that of its neighbour: an insertion in the hairy gene of Drosophila.

Fasano L, Core N, Kerridge S Rouxs Arch Dev Biol. 2017; 197(8):507-512.

PMID: 28305477 DOI: 10.1007/BF00385685.

Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila.

Witsell A, Kane D, Rubin S, McVey M Genetics. 2009; 183(3):1187-93.

PMID: 19687136 PMC: 2778970. DOI: 10.1534/genetics.109.108472.

An investigation of heterochromatin domains on the fourth chromosome of Drosophila melanogaster.

Riddle N, Leung W, Haynes K, Granok H, Wuller J, Elgin S Genetics. 2008; 178(3):1177-91.

PMID: 18245350 PMC: 2278077. DOI: 10.1534/genetics.107.081828.

Exploring strategies for protein trapping in Drosophila.

Quinones-Coello A, Petrella L, Ayers K, Melillo A, Mazzalupo S, Hudson A Genetics. 2006; 175(3):1089-104.

PMID: 17179094 PMC: 1840052. DOI: 10.1534/genetics.106.065995.

Target choice determinants of the Tc1 transposon of Caenorhabditis elegans.

Ketting R, Fischer S, Plasterk R Nucleic Acids Res. 1997; 25(20):4041-7.

PMID: 9321655 PMC: 147011. DOI: 10.1093/nar/25.20.4041.

References

Williamson V, Young E, Ciriacy M . Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II. Cell. 1981; 23(2):605-14. DOI: 10.1016/0092-8674(81)90156-2. View

Snyder M, Kimbrell D, Hunkapiller M, Hill R, Fristrom J, Davidson N . A transposable element that splits the promoter region inactivates a Drosophila cuticle protein gene. Proc Natl Acad Sci U S A. 1982; 79(23):7430-4. PMC: 347353. DOI: 10.1073/pnas.79.23.7430. View

Toh-E A, Kaneko Y, Akimaru J, Oshima Y . An insertion mutation associated with constitutive expression of repressible acid phosphatase in Saccharomyces cerevisiae. Mol Gen Genet. 1983; 191(3):339-46. DOI: 10.1007/BF00425743. View

Karess R, Rubin G . Analysis of P transposable element functions in Drosophila. Cell. 1984; 38(1):135-46. DOI: 10.1016/0092-8674(84)90534-8. View

Kidwell M, Kidwell J, Sved J . Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977; 86(4):813-33. PMC: 1213713. DOI: 10.1093/genetics/86.4.813. View

Tsubota S, Fristrom J . Genetic and biochemical properties of revertants at the rudimentary locus in Drosophila melanogaster. Mol Gen Genet. 1981; 183(2):270-6. DOI: 10.1007/BF00270628. View

Chovnick A, Gelbart W, McCarron M, Osmond B . Organization of the rosy locus in Drosophila melanogaster: evidence for a control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1976; 84(2):233-55. PMC: 1213574. DOI: 10.1093/genetics/84.2.233. View

Maniatis T, Hardison R, Lacy E, Lauer J, OConnell C, Quon D . The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978; 15(2):687-701. DOI: 10.1016/0092-8674(78)90036-3. View

Bingham P, Kidwell M, Rubin G . The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell. 1982; 29(3):995-1004. DOI: 10.1016/0092-8674(82)90463-9. View

10.

Roeder G, Fink G . DNA rearrangements associated with a transposable element in yeast. Cell. 1980; 21(1):239-49. DOI: 10.1016/0092-8674(80)90131-2. View

11.

Payne G, Bishop J, Varmus H . Multiple arrangements of viral DNA and an activated host oncogene in bursal lymphomas. Nature. 1982; 295(5846):209-14. DOI: 10.1038/295209a0. View

12.

Falk D . Genetic mosaics of the rudimentary locus of Drosophila melanogaster: a genetical investigation into the physiology of pyrimidine synthesis. Dev Biol. 1977; 58(1):134-47. DOI: 10.1016/0012-1606(77)90080-x. View

13.

McCLINTOCK B . Chromosome organization and genic expression. Cold Spring Harb Symp Quant Biol. 1951; 16:13-47. DOI: 10.1101/sqb.1951.016.01.004. View

14.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

15.

Neel B, Hayward W, Robinson H, Fang J, Astrin S . Avian leukosis virus-induced tumors have common proviral integration sites and synthesize discrete new RNAs: oncogenesis by promoter insertion. Cell. 1981; 23(2):323-34. DOI: 10.1016/0092-8674(81)90128-8. View

16.

Goldberg D . Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1980; 77(10):5794-8. PMC: 350157. DOI: 10.1073/pnas.77.10.5794. View

17.

Hayward W, Neel B, Astrin S . Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis. Nature. 1981; 290(5806):475-80. DOI: 10.1038/290475a0. View

18.

Segraves W, Louis C, Tsubota S, Schedl P, Rawls J, Jarry B . The rudimentary locus of Drosophila melanogaster. J Mol Biol. 1984; 175(1):1-17. DOI: 10.1016/0022-2836(84)90441-8. View

19.

Brothers V, Tsubota S, Germeraad S, Fristrom J . Rudimentary locus of Drosophila melanogaster: partial purification of a carbamylphosphate synthase--aspartate transcarbamylase--dihydroorotase complex. Biochem Genet. 1978; 16(3-4):321-32. DOI: 10.1007/BF00484088. View

20.

McGinnis W, Shermoen A, Beckendorf S . A transposable element inserted just 5' to a Drosophila glue protein gene alters gene expression and chromatin structure. Cell. 1983; 34(1):75-84. DOI: 10.1016/0092-8674(83)90137-x. View