OnlyParahydrogen SpectrosopY (OPSY) Pulse Sequences - One Does Not Fit All

The hyperpolarization of nuclear spins using parahydrogen is an interesting effect that allows to increase the magnetic resonance signal by several orders of magnitude. Known as ParaHydrogen And Synthesis Allow Dramatically Enhanced Nuclear Alignment (PASADENA) and ParaHydrogen Induced Polarization (PHIP), the method was successfully used for in vitro analysis and in vivo imaging. In this contribution, we investigated four known and four new variants of Only Parahydrogen SpectroscopY (OPSY) sequences (Aguilar et al., 2007) with respect to the selective preparation of hyperpolarized NMR signal and background suppression. Depending on the method chosen, either anti-phase, in-phase or a mixture of both signals are obtained: anti-phase signals are beneficial to identify hyperpolarized signals and the structure or J-coupling constants; in-phase signals are useful for imaging applications or when the lines are broad. This comprehensive overview of sequences new and old facilitates selecting the right sequence for the task at hand.