Organization and Nucleotide Sequence of Genes Encoding Core Components of the Phycobilisomes from Synechococcus 6301

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1986 Dec 1

PMID 3031427

Citations 19

Authors

J Houmard

D Mazel

C Moguet

D A Bryant

N Tandeau de Marsac

Affiliations

Soon will be listed here.

Abstract

Cyanobacteria possess specialized organelles, called phycobilisomes, which collect and transfer light energy to the reaction centres of photosystem II, in the photosynthetic membrane. Phycobilisomes consist of a central core, mainly composed of allophycocyanin, from which six rods radiate. We report here the isolation, for the first time, of three genes that encode core components of cyanobacterial phycobilisomes. The genes coding for the alpha- and beta-subunit apoproteins of allophycocyanin (apcA and apcB) were cloned from Synechococcus PCC 6301 and subjected to nucleotide sequence analysis. Dowstream of apcB, we found a third open reading frame (apcC) which, by comparison with known amino acid sequences, was assigned to L7.8c, a linker polypeptide associated with phycobiliproteins within the core of the phycobilisomes. Homologies between amino acid sequences deduced from the nucleotide sequence of the Synechococcus PCC 6301 apc genes and the amino acid sequences published for corresponding proteins either from cyanobacteria or chloroplast-like organelles of eukaryotic organisms, are 75% or more. The genetic organization of this photosynthetic gene cluster relative to that observed in the cyanelle genome of the flagellate Cyanophora paradoxa is discussed.

Citing Articles

A multigene family in Calothrix sp. PCC 7601 encodes phycocyanin, the major component of the cyanobacterial light-harvesting antenna.

Mazel D, Houmard J, de Marsac N Mol Gen Genet. 2017; 211(2):296-304.

PMID: 28124876 DOI: 10.1007/BF00330607.

Characterization of phycobiliprotein and linker polypeptide genes in Fremyella diplosiphon and their regulated expression during complementary chromatic adaptation.

Grossman A, Lemaux P, Conley P, Bruns B, Anderson L Photosynth Res. 2014; 17(1-2):23-56.

PMID: 24429660 DOI: 10.1007/BF00047680.

Photoregulation of gene expression in the filamentous cyanobacterium Calothrix sp. PCC 7601: light-harvesting complexes and cell differentiation.

De Marsac N, Mazel D, Damerval T, Guglielmi G, Capuano V, Houmard J Photosynth Res. 2014; 18(1-2):99-132.

PMID: 24425162 DOI: 10.1007/BF00042981.

Photoreversibility of the Effect of Red and Green Light Pulses on the Accumulation in Darkness of mRNAs Coding for Phycocyanin and Phycoerythrin in Fremyella diplosiphon.

Oelmuller R, Grossman A, Briggs W Plant Physiol. 1988; 88(4):1084-91.

PMID: 16666426 PMC: 1055720. DOI: 10.1104/pp.88.4.1084.

Changes in Accumulation and Synthesis of Transcripts Encoding Phycobilisome Components during Acclimation of Fremyella diplosiphon to Different Light Qualities.

Oelmuller R, Conley P, Federspiel N, Briggs W, Grossman A Plant Physiol. 1988; 88(4):1077-83.

PMID: 16666425 PMC: 1055719. DOI: 10.1104/pp.88.4.1077.

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