Characterization of Phycobiliprotein and Linker Polypeptide Genes in Fremyella Diplosiphon and Their Regulated Expression During Complementary Chromatic Adaptation

Overview

Journal Photosynth Res

Publisher Springer

Date 2014 Jan 17

PMID 24429660

Citations 7

Authors

A R Grossman

P G Lemaux

P B Conley

B U Bruns

L K Anderson

Affiliations

Soon will be listed here.

Abstract

Phycobilisomes, comprised of both chromophoric (phycobiliproteins) and non-chromophoric (linker polypeptides) proteins, are light-harvesting complexes present in the prokaryotic cyanobacteria and the eukaryotic red algae. Many cyanobacteria exhibit complementary chromatic adaptation, a process which enables these organisms to optimize absorption of prevalent wavelengths of light by altering the composition of the phycobilisome. To examine the mechanisms involved in adjusting the levels of phycobilisome components during complementary chromatic adaptation, we have isolated and sequenced genes encoding phycobiliprotein and linker polypeptides in the cyanobacterium Fremyella diplosiphon, analyzed their transcriptional characteristics (transcript sizes and abundance when F. diplosiphon is grown in different light qualities) and mapped transcript initiation and termination sites. Our results demonstrate that genes encoding phycobilisome components are often cotranscribed as polycistronic messenger RNAs. Light quality regulates the composition of the phycobilisome by causing changes in the abundance of transcripts encoding specific components, suggesting that regulation is at the level of transcription (although not eliminating the possibility of changes in mRNA stability). The work presented here sets the foundation for analyzing the evolution of the different phycobilisome components and exploring signal transduction from photoperception to activation of specific genes using in vivo and in vitro genetic technology.

Citing Articles

Organization and transcription of the genes encoding two differentially expressed phycocyanins in the cyanobacterium Pseudanabaena sp. PCC 7409.

Dubbs J, Bryant D Photosynth Res. 2013; 36(3):169-83.

PMID: 24318921 DOI: 10.1007/BF00033036.

Effect of glucose and light-dark environment on pigmentation profiles in the cyanobacterium Calothrix elenkenii.

Prasanna R, Pabby A, Singh P Folia Microbiol (Praha). 2004; 49(1):26-30.

PMID: 15114861 DOI: 10.1007/BF02931641.

Cloning of the phycobilisome rod linker genes from the cyanobacterium Synechococcus sp. PCC 6301 and their inactivation in Synechococcus sp. PCC 7942.

Bhalerao R, Lind L, PERSSON C, Gustafsson P Mol Gen Genet. 1993; 237(1-2):89-96.

PMID: 8455571 DOI: 10.1007/BF00282788.

Characterization and transcript analysis of the major phycobiliprotein subunit genes from Aglaothamnion neglectum (Rhodophyta).

Apt K, Grossman A Plant Mol Biol. 1993; 21(1):27-38.

PMID: 7678762 DOI: 10.1007/BF00039615.

Light-harvesting proteins of diatoms: their relationship to the chlorophyll a/b binding proteins of higher plants and their mode of transport into plastids.

Grossman A, Manodori A, Snyder D Mol Gen Genet. 1990; 224(1):91-100.

PMID: 2277634 DOI: 10.1007/BF00259455.

References

Riethman H, Mawhinney T, Sherman L . Phycobilisome-associated glycoproteins in the cyanobacterium Anacystis nidulans R 2. FEBS Lett. 1987; 215(2):209-14. DOI: 10.1016/0014-5793(87)80147-3. View

Bryant D, de Lorimier R, Lambert D, Dubbs J, Stirewalt V, Stevens Jr S . Molecular cloning and nucleotide sequence of the alpha and beta subunits of allophycocyanin from the cyanelle genome of Cyanophora paradoxa. Proc Natl Acad Sci U S A. 1985; 82(10):3242-6. PMC: 397751. DOI: 10.1073/pnas.82.10.3242. View

Lipman D, Pearson W . Rapid and sensitive protein similarity searches. Science. 1985; 227(4693):1435-41. DOI: 10.1126/science.2983426. View

Loffelhardt W, Mucke H, Crouse E, Bohnert H . Comparison of the cyanelle DNA from two different strains of Cyanophora paradoxa. Curr Genet. 2013; 7(2):139-44. DOI: 10.1007/BF00365639. View

Singh H, Sen R, Baltimore D, Sharp P . A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature. 1986; 319(6049):154-8. DOI: 10.1038/319154a0. View

Sen R, Baltimore D . Multiple nuclear factors interact with the immunoglobulin enhancer sequences. Cell. 1986; 46(5):705-16. DOI: 10.1016/0092-8674(86)90346-6. View

Bennett A, Bogorad L . Complementary chromatic adaptation in a filamentous blue-green alga. J Cell Biol. 1973; 58(2):419-35. PMC: 2109051. DOI: 10.1083/jcb.58.2.419. View

de Lorimier R, Bryant D, Porter R, Liu W, Jay E, Stevens Jr S . Genes for the alpha and beta subunits of phycocyanin. Proc Natl Acad Sci U S A. 1984; 81(24):7946-50. PMC: 392270. DOI: 10.1073/pnas.81.24.7946. View

Bryant D . The photoregulated expression of multiple phycocyanin species. A general mechanism for the control of phycocyanin synthesis in chromatically adapting cyanobacteria. Eur J Biochem. 1981; 119(2):425-9. DOI: 10.1111/j.1432-1033.1981.tb05625.x. View

10.

Conley P, Lemaux P, Grossman A . Cyanobacterial light-harvesting complex subunits encoded in two red light-induced transcripts. Science. 1985; 230(4725):550-3. DOI: 10.1126/science.3931221. View

11.

Lonneborg A, Lind L, Kalla S, Gustafsson P, Oquist G . Acclimation Processes in the Light-Harvesting System of the Cyanobacterium Anacystis nidulans following a Light Shift from White to Red Light. Plant Physiol. 1985; 78(1):110-4. PMC: 1064686. DOI: 10.1104/pp.78.1.110. View

12.

Lundell D, Glazer A . Molecular architecture of a light-harvesting antenna. Quaternary interactions in the Synechococcus 6301 phycobilisome core as revealed by partial tryptic digestion and circular dichroism studies. J Biol Chem. 1983; 258(14):8708-13. View

13.

Wolk C, Vonshak A, Kehoe P, Elhai J . Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria. Proc Natl Acad Sci U S A. 1984; 81(5):1561-5. PMC: 344877. DOI: 10.1073/pnas.81.5.1561. View

14.

Van Dyke M, Dervan P . Methidiumpropyl-EDTA.Fe(II) and DNase I footprinting report different small molecule binding site sizes on DNA. Nucleic Acids Res. 1983; 11(16):5555-67. PMC: 326297. DOI: 10.1093/nar/11.16.5555. View

15.

Vogelmann T, Scheibe J . Action spectra for chromatic adaptation in the blue-green alga Fremyella diplosiphon. Planta. 2014; 143(3):233-9. DOI: 10.1007/BF00391993. View

16.

Sherman L, Van de Putte P . Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans. J Bacteriol. 1982; 150(1):410-3. PMC: 220131. DOI: 10.1128/jb.150.1.410-413.1982. View

17.

Wu C . An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts. Nature. 1985; 317(6032):84-7. DOI: 10.1038/317084a0. View

18.

Bashford D, Chothia C, Lesk A . Determinants of a protein fold. Unique features of the globin amino acid sequences. J Mol Biol. 1987; 196(1):199-216. DOI: 10.1016/0022-2836(87)90521-3. View

19.

De Marsac N, COHEN-BAZIRE G . Molecular composition of cyanobacterial phycobilisomes. Proc Natl Acad Sci U S A. 1977; 74(4):1635-9. PMC: 430846. DOI: 10.1073/pnas.74.4.1635. View

20.

Cobley J, Miranda R . Mutations affecting chromatic adaptation in the cyanobacterium Fremyella diplosiphon. J Bacteriol. 1983; 153(3):1486-92. PMC: 221800. DOI: 10.1128/jb.153.3.1486-1492.1983. View