Basal Constriction During Midbrain-hindbrain Boundary Morphogenesis is Mediated by Wnt5b and Focal Adhesion Kinase

Overview

Journal Biol Open

Specialty Biology

Date 2018 Oct 12

PMID 30305282

Citations 13

Authors

Jennifer H Gutzman

Ellie Graeden

Isabel Brachmann

Sayumi Yamazoe

James K Chen

Hazel Sive

Affiliations

Soon will be listed here.

Abstract

Basal constriction occurs at the zebrafish midbrain-hindbrain boundary constriction (MHBC) and is likely a widespread morphogenetic mechanism. 3D reconstruction demonstrates that MHBC cells are wedge-shaped, and initially constrict basally, with subsequent apical expansion. is expressed in the MHB and is required for basal constriction. Consistent with a requirement for this pathway, expression of dominant negative Gsk3β overcomes knockdown. Immunostaining identifies focal adhesion kinase (Fak) as active in the MHB region, and knockdown demonstrates Fak is a regulator of basal constriction. Tissue specific knockdown further indicates that Fak functions cell autonomously within the MHBC. Fak acts downstream of , suggesting that Wnt5b signals locally as an early step in basal constriction and acts together with more widespread Fak activation. This study delineates signaling pathways that regulate basal constriction during brain morphogenesis.

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