Escherichia Coli Rep Gene: Sequence of the Gene, the Encoded Helicase, and Its Homology with UvrD

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Jan 26

PMID 3029683

Citations 24

Authors

C A Gilchrist

D T Denhardt

Affiliations

Soon will be listed here.

Abstract

The sequence of a 2.67-kilobase section of the Escherichia coli chromosome that contains the rep gene has been determined. This gene codes for a protein of predicted Mr 72,800, a DNA helicase, which is also a single-stranded DNA-dependent ATPase. The sequenced region contains an open reading frame of the correct length and orientation to encode the Rep protein. A secondary structure for the protein can be formulated from the amino acid sequence. We have compared both the primary and the secondary structures of Rep with other proteins and find the greatest homology between Rep and E. coli helicase II, the product of the uvrD gene.

Citing Articles

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DNA Helicases.

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PriA participates in nascent DNA synthesis in Escherichia coli.

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UvrD303, a hyperhelicase mutant that antagonizes RecA-dependent SOS expression by a mechanism that depends on its C terminus.

Centore R, Leeson M, Sandler S J Bacteriol. 2008; 191(5):1429-38.

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Viability of rep recA mutants depends on their capacity to cope with spontaneous oxidative damage and on the DnaK chaperone protein.

Bredeche M, Ehrlich S, Michel B J Bacteriol. 2001; 183(7):2165-71.

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