Differential Expression and Methylation of Integrin Subunit Alpha 11 and Thrombospondin in the Amnion of Preterm Birth

Overview

Journal Obstet Gynecol Sci

Specialty Gynecology & Obstetrics

Date 2018 Sep 27

PMID 30254992

Citations 6

Authors

Jae Young Yoo

Young-Ah You

Eun Jin Kwon

Mi Hye Park

Sunah Shim

Young Ju Kim

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to investigate the association between preterm birth and epigenetic mechanisms in the amnion.

Methods: We examined the association between differentially methylated regions (DMRs) and differentially expressed genes (DEG) using a cytosine-phosphate-guanine methylation array and whole-transcriptome sequencing from the amnion (preterm birth, n=5; full term, n=5). We enrolled 35 participants for mRNA expression analysis and pyrosequencing: 16 full-term and 19 preterm subjects. We compared the association of integrin subunit alpha 11 () and thrombospondin 2 () gene methylation status with mRNA expression in the amnion.

Results: In the preterm birth group, methylation of and genes was significantly lower ( gene: 60.30% vs. 73.16%, <0.05; gene: 64.59% vs. 73.16%, <0.05), and the expression of the genes was significantly higher than that in the full-term group ( gene: 14.20 vs. 1.57, <0.01; gene: 1.18 vs. 10.34, <0.05).

Conclusion: Methylation of the and genes in the amnion was associated with preterm birth. Thus, and gene methylation status in the amnion may be valuable in explaining the mechanism underlying preterm birth.

Citing Articles

Identification of Indicators for Preterm Birth Using Retinoid Metabolites.

You Y, Hwang S, Kim S, Park S, Lee G, Park S Metabolites. 2021; 11(7).

PMID: 34357337 PMC: 8304766. DOI: 10.3390/metabo11070443.

Integrative analysis of transcriptomic data for identification of T-cell activation-related mRNA signatures indicative of preterm birth.

Yoo J, Hyeon D, Shin Y, Kim S, You Y, Kim D Sci Rep. 2021; 11(1):2392.

PMID: 33504832 PMC: 7841165. DOI: 10.1038/s41598-021-81834-z.

Fetal Membrane Epigenetics.

Zakar T, Paul J Front Physiol. 2020; 11:588539.

PMID: 33343389 PMC: 7744818. DOI: 10.3389/fphys.2020.588539.

Racial and ethnic representation in epigenomic studies of preterm birth: a systematic review.

Collier A, Ledyard R, Montoya-Williams D, Qiu M, Dereix A, Farrokhi M Epigenomics. 2020; 13(21):1735-1746.

PMID: 33264049 PMC: 8579936. DOI: 10.2217/epi-2020-0007.

Cervicovaginal fluid cytokines as predictive markers of preterm birth in symptomatic women.

Park S, You Y, Yun H, Choi S, Hwang H, Choi S Obstet Gynecol Sci. 2020; 63(4):455-463.

PMID: 32550740 PMC: 7393743. DOI: 10.5468/ogs.19131.

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