Robust Single-cell DNA Methylome Profiling with SnmC-seq2

Overview

Journal Nat Commun

Specialty Biology

Date 2018 Sep 22

PMID 30237449

Citations 84

Authors

Chongyuan Luo

Angeline Rivkin

Jingtian Zhou

Justin P Sandoval

Laurie Kurihara

Jacinta Lucero

Rosa Castanon

Joseph R Nery

Antonio Pinto-Duarte

Brian Bui

Conor Fitzpatrick

Carolyn OConnor

Seth Ruga

Marc E Van Eden

David A Davis

Deborah C Mash

M Margarita Behrens

Joseph R Ecker

Affiliations

Soon will be listed here.

Abstract

Single-cell DNA methylome profiling has enabled the study of epigenomic heterogeneity in complex tissues and during cellular reprogramming. However, broader applications of the method have been impeded by the modest quality of sequencing libraries. Here we report snmC-seq2, which provides improved read mapping, reduced artifactual reads, enhanced throughput, as well as increased library complexity and coverage uniformity compared to snmC-seq. snmC-seq2 is an efficient strategy suited for large-scale single-cell epigenomic studies.

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