A Conserved Human Germline V Kappa Gene Directly Encodes Rheumatoid Factor Light Chains

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1986 Dec 1

PMID 3023521

Citations 49

Authors

V Radoux

P P Chen

J A Sorge

D A Carson

Affiliations

Soon will be listed here.

Abstract

The full-length gene that encodes the light chain variable regions of an idiotypically related group of human IgM kappa rheumatoid factors (RFs) has been cloned and sequenced. The deduced amino acid sequence is identical to four separate RF proteins. These results prove that genes capable of encoding human anti-IgG autoantibody light chains without any somatic mutation are present in the kappa gene repertoire of normal people.

Citing Articles

Somatic hypermutations confer rheumatoid factor activity in hepatitis C virus-associated mixed cryoglobulinemia.

Charles E, Orloff M, Nishiuchi E, Marukian S, Rice C, Dustin L Arthritis Rheum. 2013; 65(9):2430-40.

PMID: 23754128 PMC: 4026862. DOI: 10.1002/art.38041.

B cell receptor light chain repertoires show signs of selection with differences between groups of healthy individuals and SLE patients.

Schoettler N, Ni D, Weigert M Mol Immunol. 2012; 51(3-4):273-82.

PMID: 22516082 PMC: 4264353. DOI: 10.1016/j.molimm.2012.03.028.

Rheumatoid factors induce signaling from B cells, leading to Epstein-Barr virus and B-cell activation.

Yang L, Hakoda M, Iwabuchi K, Takeda T, Koike T, Kamatani N J Virol. 2004; 78(18):9918-23.

PMID: 15331725 PMC: 515018. DOI: 10.1128/JVI.78.18.9918-9923.2004.

Recombinant monoclonal thyrotropin-stimulation blocking antibody (TSBAb) established from peripheral lymphocytes of a hypothyroid patient with primary myxedema.

Moriyama K, Okuda J, Saijo M, Hattori Y, Kanamoto N, Hataya Y J Endocrinol Invest. 2004; 26(11):1076-80.

PMID: 15008244 DOI: 10.1007/BF03345253.

The antigen-binding domain of a human IgG-anti-F(ab')2 autoantibody.

Welschof M, Terness P, Kipriyanov S, Stanescu D, Breitling F, Dorsam H Proc Natl Acad Sci U S A. 1997; 94(5):1902-7.

PMID: 9050877 PMC: 20015. DOI: 10.1073/pnas.94.5.1902.

References

Goni F, Chen P, Pons-Estel B, Carson D, Frangione B . Sequence similarities and cross-idiotypic specificity of L chains among human monoclonal IgM kappa with anti-gamma-globulin activity. J Immunol. 1985; 135(6):4073-9. View

Fong S, Chen P, Gilbertson T, Weber J, Fox R, Carson D . Expression of three cross-reactive idiotypes on rheumatoid factor autoantibodies from patients with autoimmune diseases and seropositive adults. J Immunol. 1986; 137(1):122-8. View

Naparstek Y, Andre-Schwartz J, Manser T, Wysocki L, Breitman L, Stollar B . A single germline VH gene segment of normal A/J mice encodes autoantibodies characteristic of systemic lupus erythematosus. J Exp Med. 1986; 164(2):614-26. PMC: 2188235. DOI: 10.1084/jem.164.2.614. View

KUNKEL H, Agnello V, Joslin F, Winchester R, Capra J . Cross-idiotypic specificity among monoclonal IgM proteins with anti- -globulin activity. J Exp Med. 1973; 137(2):331-42. PMC: 2139491. DOI: 10.1084/jem.137.2.331. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Chen P, Goni F, Houghten R, Fong S, Goldfien R, Vaughan J . Characterization of human rheumatoid factors with seven antiidiotypes induced by synthetic hypervariable region peptides. J Exp Med. 1985; 162(2):487-500. PMC: 2187757. DOI: 10.1084/jem.162.2.487. View

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

Frischauf A, Lehrach H, Poustka A, Murray N . Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983; 170(4):827-42. DOI: 10.1016/s0022-2836(83)80190-9. View

Diamond B, Scharff M . Somatic mutation of the T15 heavy chain gives rise to an antibody with autoantibody specificity. Proc Natl Acad Sci U S A. 1984; 81(18):5841-4. PMC: 391807. DOI: 10.1073/pnas.81.18.5841. View

10.

Yancopoulos G, Desiderio S, Paskind M, Kearney J, Baltimore D, Alt F . Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell lines. Nature. 1984; 311(5988):727-33. DOI: 10.1038/311727a0. View

11.

Chen P, Goni F, Fong S, Jirik F, Vaughan J, Frangione B . The majority of human monoclonal IgM rheumatoid factors express a "primary structure-dependent" cross-reactive idiotype. J Immunol. 1985; 134(5):3281-5. View

12.

Andrews D, Capra J . Complete amino acid sequence of variable domains from two monoclonal human anti-gamma globulins of the Wa cross-idiotypic group: suggestion that the J segments are involved in the structural correlate of the idiotype. Proc Natl Acad Sci U S A. 1981; 78(6):3799-803. PMC: 319660. DOI: 10.1073/pnas.78.6.3799. View