Partial Characterization of an Endogenous Factor Which Modulates the Effect of Catecholamines on Synaptosomal Na+, K+-ATPase

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1986 Jul 1

PMID 3018605

Citations 19

Authors

G Rodriguez de Lores Arnaiz

M Antonelli de Gomez de Lima

Affiliations

Soon will be listed here.

Abstract

We have previously presented evidence for the existence of a brain soluble factor which mediates the stimulation of synaptosomal ATPases by catecholamines. The stimulation of synaptosomal ATPases by dopamine plus brain soluble fraction was not modified if the soluble fraction was heated for 5 min at 95 degrees C. One day after preparation, the soluble factor inhibited the Na+, K+-ATPase, but not the Mg2+-ATPase activity, and subsequent addition of noradrenaline stimulated the ATPases activities. The inhibitory effect of a 24 h soluble fraction disappeared if the soluble fraction was dialyzed; in this case, noradrenaline did not activate the enzyme activities. Gel filtration in Sephadex G-50 permitted separating a subfraction which inhibited ATPase activity (peak II) from another which stimulated ATPase activity (peak I). Peak I stimulated both Na+, K+, and Mg2+ ATPases. Peak II inhibited only Na+, K+-ATPase, and when stored acidified, it mediated ATPases stimulation by noradrenaline.

Citing Articles

High-affinity neurotensin receptor is involved in phosphoinositide turnover increase by inhibition of sodium pump in neonatal rat brain.

Pereyra-Alfonso S, Del Valle Armanino M, Vazquez C, Pena C, Rodriguez de Lores Arnaiz G Neurochem Res. 2008; 33(11):2206-13.

PMID: 18758956 DOI: 10.1007/s11064-008-9672-2.

The expression of NMDA receptor subunits in cerebral cortex and hippocampus is differentially increased by administration of endobain E, a Na+, K+-ATPase inhibitor.

Bersier M, Pena C, Rodriguez de Lores Arnaiz G Neurochem Res. 2007; 33(1):66-72.

PMID: 17680361 DOI: 10.1007/s11064-007-9412-z.

Modulation of aspartate release by ascorbic acid and endobain E, an endogenous Na+, K+ -ATPase inhibitor.

Bersier M, Miksztowicz V, Pena C, Rodriguez de Lores Arnaiz G Neurochem Res. 2005; 30(4):479-86.

PMID: 16076019 DOI: 10.1007/s11064-005-2684-2.

The effect of endogenous modulator endobain E on NMDA receptor is interfered by Zn2+ but is independent of modulation by spermidine.

Reines A, Zarate S, Pena C, Rodriguez de Lores Arnaiz G Neurochem Res. 2004; 29(4):819-25.

PMID: 15098947 DOI: 10.1023/b:nere.0000018856.99773.71.

The effect of an endogenous Na+, K+-ATPase inhibitor on rat lens transparency and ultrastructure.

Pellegrino de Iraldi A, Pena C, Rodriguez de Lores Arnaiz G Cell Mol Neurobiol. 2003; 23(2):131-41.

PMID: 12735627 PMC: 11530163. DOI: 10.1023/a:1022941720546.

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