Kinetics of K(+)-p-nitrophenyl Phosphatase Stimulation by a Brain Soluble Fraction

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1997 Feb 1

PMID 9016848

Authors

G Rodriguez de Lores Arnaiz

Affiliations

Soon will be listed here.

Abstract

We have already described the separation of two brain soluble fractions by Sephadex G-50, one of which stimulates (peak I) and the other inhibits (peak II) Na+, K(+)-ATPase and K(+)-p-nitrophenylphosphatase (K(+)-p-NPPase) activities. Here we examine the features of synaptosomal membrane p-NPPase activity in the presence and absence of brain peak I. It was observed that stimulation of Mg2+, K(+)-p-NPPase activity by peak I was concentration dependent. The ability of peak I to stimulate p-NPPase activity was lost by heat treatment followed by brief centrifugation. Pure serum albumin also stimulated enzyme activity. K(+)-p-NPPase stimulation by peak I proved dependent on K+ concentration but independent of Mg2+ and substrate p-nitrophenylphosphate concentrations. Since our determinations were performed in a non-phosphorylating condition reflecting the Na+, K(+)-ATPase Na+ site, it is suggested that peak I may stimulate the Na+-dependent enzyme phosphorylation known to take place from the internal cytoplasmic side.

References

Poduslo J, Curran G . Increased permeability across the blood-nerve barrier of albumin glycated in vitro and in vivo from patients with diabetic polyneuropathy. Proc Natl Acad Sci U S A. 1992; 89(6):2218-22. PMC: 48628. DOI: 10.1073/pnas.89.6.2218. View

Rodriguez de Lores Arnaiz G . An endogenous factor which interacts with synaptosomal membrane Na+, K(+)-ATPase activation by K+. Neurochem Res. 1993; 18(6):655-61. DOI: 10.1007/BF00966778. View

Nowicki S, Enero M, Rodriguez de Lores Arnaiz G . Diuretic and natriuretic effect of a brain soluble fraction that inhibits neuronal Na+,K(+)-ATPase. Life Sci. 1990; 47(13):1091-8. DOI: 10.1016/0024-3205(90)90167-p. View

Antonelli M, Casillas T, Rodriguez de Lores Arnaiz G . Effect of Na+, K(+)-ATPase modifiers on high-affinity ouabain binding determined by quantitative autoradiography. J Neurosci Res. 1991; 28(3):324-31. DOI: 10.1002/jnr.490280303. View

Stahl W . The Na,K-ATPase of nervous tissue. Neurochem Int. 2010; 8(4):449-76. DOI: 10.1016/0197-0186(86)90179-8. View

Skou J . Effect of ATP on the intermediary steps of the reaction of the (Na+ plusK+)-dependent enzyme system. I. Studied by the use of N-ethylmaleimide inhibition as a tool. Biochim Biophys Acta. 1974; 339(2):234-45. DOI: 10.1016/0005-2736(74)90321-6. View

Swann A, Albers R . Sodium and potassium ion-dependent adenosine triphosphatase of mammalian brain. Interactions of magnesium ions with the phosphatase site. Biochim Biophys Acta. 1978; 523(1):215-27. DOI: 10.1016/0005-2744(78)90024-4. View

Rodriguez de Lores Arnaiz G, PELLEGRINO DE IRALDI A . The release of catecholamines by an endogenous factor that inhibits neuronal Na+,K(+)-ATPase. Microsc Electron Biol Celular. 1991; 15(1):93-105. View

Rodriguez De Lores A, Alberici M, De Robertis E . Ultrastructural and enzymic studies of cholinergic and non-cholinergic synaptic membranes isolated from brain cortex. J Neurochem. 1967; 14(2):215-25. DOI: 10.1111/j.1471-4159.1967.tb05897.x. View

10.

Tildon J, McKenna M, Stevenson J . Differential effects of serum protein(s) on substrate oxidation by isolated synaptosomes and cultured rat brain astrocytes. Dev Neurosci. 1993; 15(3-5):226-32. DOI: 10.1159/000111338. View

11.

Albers R, RODRIGUEZDE LORES , DeROBERTIS E . SODIUM-POTASSIUM-ACTIVATED ATPASE AND POTASSIUM-ACTIVATED P-NITROPHENYLPHOSPHATASE: A COMPARISON OF THEIR SUBCELLULAR LOCALIZATIONS IN RAT BRAIN. Proc Natl Acad Sci U S A. 1965; 53:557-64. PMC: 336976. DOI: 10.1073/pnas.53.3.557. View

12.

Robinson J . Kinetic studies on a brain microsomal adenosine triphosphatase. II. Potassium-dependent phosphatase activity. Biochemistry. 1969; 8(8):3348-55. DOI: 10.1021/bi00836a032. View

13.

Rodriguez de Lores Arnaiz G, de Gomez de Lima M . The aging of a brain soluble fraction modifies its effect on the activity of neuronal Na+, K+-ATPase. Life Sci. 1988; 42(26):2683-9. DOI: 10.1016/0024-3205(88)90244-5. View

14.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

15.

Kairane C, Zilmer M, Mutt V, Sillard R . Activation of Na,K-ATPase by an endogenous peptide, PEC-60. FEBS Lett. 1994; 345(1):1-4. DOI: 10.1016/0014-5793(94)00407-2. View

16.

Das P, Chakrabarti R, Bandopadhyay S, Ray T . Demonstration of an endogenous activator for the Na+, K(+)-ATPase system. Mol Cell Biochem. 1989; 91(1-2):123-9. DOI: 10.1007/BF00228087. View

17.

Rodriguez de Lores Arnaiz G, Pena C . Characterization of synaptosomal membrane Na+, K(+)-ATPase inhibitors. Neurochem Int. 1995; 27(4-5):319-27. DOI: 10.1016/0197-0186(95)00013-x. View

18.

Rodriguez de Lores Arnaiz G, Antonelli de Gomez de Lima M, Girardi E . Different properties of two brain extracts separated in Sephadex G-50 that modify synaptosomal ATPase activities. Neurochem Res. 1988; 13(3):229-35. DOI: 10.1007/BF00971538. View

19.

Rodriguez de Lores Arnaiz G . In search of synaptosomal Na+,K(+)-ATPase regulators. Mol Neurobiol. 1992; 6(4):359-75. DOI: 10.1007/BF02757941. View

20.

Rodriguez de Lores Arnaiz G, Antonelli de Gomez de Lima M . Partial characterization of an endogenous factor which modulates the effect of catecholamines on synaptosomal Na+, K+-ATPase. Neurochem Res. 1986; 11(7):933-47. DOI: 10.1007/BF00965584. View