The Inhibitory Activity of a Brain Extract on Synaptosomal Na+, K+-ATPase is Sensitive to Carboxypeptidase A and to Chelating Agents

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1988 Mar 1

PMID 2838764

Citations 4

Authors

M Antonelli de Gomez de Lima

G Rodriquez de Lores Arnaiz

Affiliations

Soon will be listed here.

Abstract

In the present study some properties of an inhibitory extract of synaptosomal membrane Na+, K+-ATPase were investigated. This extract (peak II) was prepared by gel filtration in Sephadex G-50 of a soluble fraction of the rat cerebral cortex. Ultrafiltration of peak II through Amicon membranes indicated that the inhibitor has a low MW (less than 1000). The inhibitory activity was not modified by heating in neutral pH at 95 degrees C for 20 min but it was destroyed by charring in acid pH at 200 degrees C for 120 min. The inhibitory activity decreased by incubation of peak II with carboxypeptidase A. These findings suggest that the factor responsible for the inhibition of Na+, K+-ATPase activity is probably a polypeptide. On the other hand, the inhibition was reverted by the chelators EDTA and EGTA, indicating the participation of an ionic compound as well. The increase of Mg2+ concentration during the enzyme assay did not increase the inhibition, indicating that the ion involved might not be vanadate. It is suggested that both a polypeptide and an ionic compound coparticipate in the inhibitory effect of peak II on Na+, K+-ATPase activity.

Citing Articles

An endogenous factor which interacts with synaptosomal membrane Na+, K(+)-ATPase activation by K+.

Rodriguez de Lores Arnaiz G Neurochem Res. 1993; 18(6):655-61.

PMID: 8389989 DOI: 10.1007/BF00966778.

Different properties of two brain extracts separated in Sephadex G-50 that modify synaptosomal ATPase activities.

Rodriguez de Lores Arnaiz G, Antonelli de Gomez de Lima M, Girardi E Neurochem Res. 1988; 13(3):229-35.

PMID: 2455236 DOI: 10.1007/BF00971538.

Effect of tissue specificity of brain soluble fractions on Na+, K(+)-ATPase activity.

Rodriguez de Lores Arnaiz G Neurochem Res. 1990; 15(3):289-94.

PMID: 2164167 DOI: 10.1007/BF00968674.

In search of synaptosomal Na+,K(+)-ATPase regulators.

Rodriguez de Lores Arnaiz G Mol Neurobiol. 1992; 6(4):359-75.

PMID: 1363048 DOI: 10.1007/BF02757941.

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