Modified Aptamers Enable Quantitative Sub-10-nm Cellular DNA-PAINT Imaging

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2018 Aug 22

PMID 30127504

Citations 60

Authors

Sebastian Strauss

Philipp C Nickels

Maximilian T Strauss

Vilma Jimenez Sabinina

Jan Ellenberg

Jeffrey D Carter

Shashi Gupta

Nebojsa Janjic

Ralf Jungmann

Affiliations

Soon will be listed here.

Abstract

Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.

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