Breaking the Diffraction Resolution Limit by Stimulated Emission: Stimulated-emission-depletion Fluorescence Microscopy

Overview

Journal Opt Lett

Specialty Ophthalmology

Date 2009 Oct 22

PMID 19844443

Citations 1298

Authors

S W Hell

J Wichmann

Affiliations

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Abstract

We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation point-spread function. In contrast to near-field scanning optical microscopy, this method can produce three-dimensional images of translucent specimens.

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