Multiple Mutations in Lipid-A Modification Pathway & Novel Variants in Colistin-resistant

Overview

Journal Future Sci OA

Specialty Biotechnology

Date 2018 Aug 17

PMID 30112189

Citations 16

Authors

Purva Mathur

Balaji Veeraraghavan

Naveen Kumar Devanga Ragupathi

Francis Yesurajan Inbanathan

Surbhi Khurana

Nidhi Bhardwaj

Subodh Kumar

Sushma Sagar

Amit Gupta

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate antimicrobial resistance mechanisms in a cluster of colistin-resistant .

Methods: Antimicrobial susceptibility was tested by disk diffusion and broth microdilution. Whole-genome sequencing and genome analysis were performed.

Results: The eight colistin-resistant isolates belonged to three different clones (ST11, 14 and 231). and genes from lipid modification pathway had novel (R157S in and Q319R in ) and rare mutations (V39L, R152H, S260L and A279G in ). Several substitutions were also identified in , , and genes. The genes were absent in all isolates. Isolates had variants from existing classes of gene.

Conclusion: Complex combination of mutations might have led to colistin resistance, which suggests that continuous surveillance of molecular mechanisms is required.

Citing Articles

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