Antiproliferative Activity of Neem Leaf Extracts Obtained by a Sequential Pressurized Liquid Extraction

Overview

Journal Pharmaceuticals (Basel)

Publisher MDPI

Specialty Chemistry

Date 2018 Aug 1

PMID 30061479

Citations 4

Authors

Klebson S Santos

Andriele M Barbosa

Victor Freitas

Ana Veruska C S Muniz

Marcelo C Mendonca

Ricardo C Calhelha

Isabel C F R Ferreira

Elton Franceschi

Francine F Padilha

Maria Beatriz P P Oliveira

Claudio Dariva

Affiliations

Soon will be listed here.

Abstract

A. Juss (neem) extracts have been used in pharmaceutical applications as antitumor agents, due to their terpenes and phenolic compounds. To obtain extracts from neem leaves with potential antiproliferative effect, a sequential process of pressurized liquid extraction was carried out in a fixed bed extractor at 25 °C and 100 bar, using hexane (SH), ethyl acetate (SEA), and ethanol (SE) as solvents. Extractions using only ethanol (EE) was also conducted to compare the characteristics of the fractionated extracts. The results obtained by liquid chromatography-electrospray ionization mass spectrometry suggested a higher concentration of terpenes in the SEA extract in comparison to SH, SE, and EE extracts. Therefore, antiproliferative activity showed that SEA extracts were the most efficient inhibitor to human tumor cells MCF-7, NCI-H460, HeLa, and HepG2. Hepatocellular cells were more resistant to SH, SEA, SE, and EE compared to breast, lung, hepatocellular, and cervical malignant cells. Neem fractioned extracts obtained in the present study seem to be more selective for malignant cells compared to the non-tumor cells.

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References

Ji X, Avula B, Khan I . Quantitative and qualitative determination of six xanthones in Garcinia mangostana L. by LC-PDA and LC-ESI-MS. J Pharm Biomed Anal. 2006; 43(4):1270-6. DOI: 10.1016/j.jpba.2006.10.018. View

Haldar S, Mulani F, Aarthy T, Dandekar D, Thulasiram H . Expedient preparative isolation and tandem mass spectrometric characterization of C-seco triterpenoids from Neem oil. J Chromatogr A. 2014; 1366:1-14. DOI: 10.1016/j.chroma.2014.09.006. View

Mahapatra S, Young C, Kohli M, Karnes R, Klee E, Holmes M . Antiangiogenic Effects and Therapeutic Targets of Azadirachta indica Leaf Extract in Endothelial Cells. Evid Based Complement Alternat Med. 2012; 2012:303019. PMC: 3296311. DOI: 10.1155/2012/303019. View

Mustafa A, Turner C . Pressurized liquid extraction as a green approach in food and herbal plants extraction: A review. Anal Chim Acta. 2011; 703(1):8-18. DOI: 10.1016/j.aca.2011.07.018. View

Savic S, Vojinovic K, Milenkovic S, Smelcerovic A, Lamshoeft M, Petronijevic Z . Enzymatic oxidation of rutin by horseradish peroxidase: kinetic mechanism and identification of a dimeric product by LC-Orbitrap mass spectrometry. Food Chem. 2013; 141(4):4194-9. DOI: 10.1016/j.foodchem.2013.07.010. View

Jiang B, Kronenberg F, Balick M, Kennelly E . Analysis of formononetin from black cohosh (Actaea racemosa). Phytomedicine. 2006; 13(7):477-86. DOI: 10.1016/j.phymed.2005.06.007. View

Sharma C, Vas A, Goala P, Gheewala T, Rizvi T, Hussain A . Ethanolic Neem (Azadirachta indica) Leaf Extract Prevents Growth of MCF-7 and HeLa Cells and Potentiates the Therapeutic Index of Cisplatin. J Oncol. 2014; 2014:321754. PMC: 3929266. DOI: 10.1155/2014/321754. View

Guimaraes R, Barros L, Duenas M, Calhelha R, Carvalho A, Santos-Buelga C . Nutrients, phytochemicals and bioactivity of wild Roman chamomile: a comparison between the herb and its preparations. Food Chem. 2012; 136(2):718-25. DOI: 10.1016/j.foodchem.2012.08.025. View

Wolfender J, Ndjoko K, Hostettmann K . Liquid chromatography with ultraviolet absorbance-mass spectrometric detection and with nuclear magnetic resonance spectroscopy: a powerful combination for the on-line structural investigation of plant metabolites. J Chromatogr A. 2003; 1000(1-2):437-55. DOI: 10.1016/s0021-9673(03)00303-0. View

10.

Hao F, Kumar S, Yadav N, Chandra D . Neem components as potential agents for cancer prevention and treatment. Biochim Biophys Acta. 2014; 1846(1):247-57. PMC: 4734358. DOI: 10.1016/j.bbcan.2014.07.002. View

11.

Pavei C, Kaiser S, Verza S, Borre G, Ortega G . HPLC-PDA method for quinovic acid glycosides assay in Cat's claw (Uncaria tomentosa) associated with UPLC/Q-TOF-MS analysis. J Pharm Biomed Anal. 2012; 62:250-7. DOI: 10.1016/j.jpba.2011.12.031. View

12.

Donno D, Mellano M, Cerutti A, Beccaro G . Biomolecules and Natural Medicine Preparations: Analysis of New Sources of Bioactive Compounds from Ribes and Rubus spp. Buds. Pharmaceuticals (Basel). 2016; 9(1). PMC: 4812371. DOI: 10.3390/ph9010007. View

13.

Patel S, Nagulapalli Venkata K, Bhattacharyya P, Sethi G, Bishayee A . Potential of neem (Azadirachta indica L.) for prevention and treatment of oncologic diseases. Semin Cancer Biol. 2016; 40-41:100-115. DOI: 10.1016/j.semcancer.2016.03.002. View

14.

Schaaf O, Jarvis A, van der Esch S, Giagnacovo G, Oldham N . Rapid and sensitive analysis of azadirachtin and related triterpenoids from Neem (Azadirachta indica) by high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. J Chromatogr A. 2000; 886(1-2):89-97. DOI: 10.1016/s0021-9673(00)00492-1. View

15.

Gika H, Theodoridis G, Plumb R, Wilson I . Current practice of liquid chromatography-mass spectrometry in metabolomics and metabonomics. J Pharm Biomed Anal. 2013; 87:12-25. DOI: 10.1016/j.jpba.2013.06.032. View

16.

Eng A, Heng M, Ong E . Evaluation of surfactant assisted pressurized liquid extraction for the determination of glycyrrhizin and ephedrine in medicinal plants. Anal Chim Acta. 2007; 583(2):289-95. DOI: 10.1016/j.aca.2006.09.019. View

17.

Smith R . Extractions with superheated water. J Chromatogr A. 2002; 975(1):31-46. DOI: 10.1016/s0021-9673(02)01225-6. View

18.

Sasidharan S, Chen Y, Saravanan D, Sundram K, Yoga Latha L . Extraction, isolation and characterization of bioactive compounds from plants' extracts. Afr J Tradit Complement Altern Med. 2012; 8(1):1-10. PMC: 3218439. View

19.

Cowan M . Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12(4):564-82. PMC: 88925. DOI: 10.1128/CMR.12.4.564. View

20.

Wu Q, Yuan H, Zhang L, Zhang Y . Recent advances on multidimensional liquid chromatography-mass spectrometry for proteomics: from qualitative to quantitative analysis--a review. Anal Chim Acta. 2012; 731:1-10. DOI: 10.1016/j.aca.2012.04.010. View