Membrane Association of the Transforming Protein of Avian Sarcoma Virus UR2 and Mutants Temperature Sensitive for Cellular Transformation and Protein Kinase Activity

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1985 Dec 1

PMID 2999433

Citations 14

Authors

E A GARBER

T Hanafusa

H Hanafusa

Affiliations

Soon will be listed here.

Abstract

The localization of the transforming protein P68gag-ros of avian sarcoma virus UR2, which has a hydrophobic region at the N terminus of its ros-specific tyrosine kinase-encoding sequence, was examined by subcellular fractionation. P68 behaved as an integral membrane protein associated with the plasma membrane of transformed cells. P68 became membrane associated very rapidly in its biogenesis. Three temperature-sensitive mutants of UR2 were isolated and characterized. Cells infected with the mutants were temperature sensitive for morphological alteration and colony formation. The mutant P68 proteins were membrane associated in mutant-infected cells regardless of the temperature but were active as protein kinases only at the permissive temperature. The results suggest that P68 is a membrane-associated protein whose kinase activity plays a crucial role in UR2-mediated cell transformation.

Citing Articles

Molecular and biochemical bases for activation of the transforming potential of the proto-oncogene c-ros.

Zong C, Poon B, Chen J, Wang L J Virol. 1993; 67(11):6453-62.

PMID: 8411348 PMC: 238081. DOI: 10.1128/JVI.67.11.6453-6462.1993.

Ala-->Gly mutation in the putative catalytic loop confers temperature sensitivity on Ros, insulin receptor, and insulin-like growth factor I receptor protein-tyrosine kinases.

Chen J, Hanafusa T, Wang L Proc Natl Acad Sci U S A. 1994; 91(1):321-5.

PMID: 8278385 PMC: 42939. DOI: 10.1073/pnas.91.1.321.

Modulatory effect of the transmembrane domain of the protein-tyrosine kinase encoded by oncogene ros: biological function and substrate interaction.

Zong C, Wang L Proc Natl Acad Sci U S A. 1994; 91(23):10982-6.

PMID: 7526386 PMC: 45150. DOI: 10.1073/pnas.91.23.10982.

The human c-ros gene (ROS) is located at chromosome region 6q16----6q22.

Nagarajan L, Louie E, Tsujimoto Y, BALDUZZI P, Huebner K, Croce C Proc Natl Acad Sci U S A. 1986; 83(17):6568-72.

PMID: 3529088 PMC: 386545. DOI: 10.1073/pnas.83.17.6568.

Activation of transforming potential of the human insulin receptor gene.

Wang L, Lin B, Jong S, Dixon D, Ellis L, Roth R Proc Natl Acad Sci U S A. 1987; 84(16):5725-9.

PMID: 3039503 PMC: 298935. DOI: 10.1073/pnas.84.16.5725.

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