Targeting CREB-binding Protein Overrides LPS Induced Radioresistance in Non-small Cell Lung Cancer Cell Lines

Overview

Journal Oncotarget

Specialty Oncology

Date 2018 Jul 11

PMID 29989005

Citations 5

Authors

Mira Y Gokyildirim

Ulrich Grandel

Katja Hattar

Gabriele Dahlem

Elena Schuetz

Florian H Leinberger

Fabian Eberle

Ulf Sibelius

Friedrich Grimminger

Werner Seeger

Rita Engenhart-Cabillic

Ekkehard Dikomey

Florentine S B Subtil

Affiliations

Soon will be listed here.

Abstract

Non-small cell lung cancer (NSCLC) has a very poor prognosis even when treated with the best therapies available today often including radiation. NSCLC is frequently complicated by pulmonary infections which appear to impair prognosis as well as therapy, whereby the underlying mechanisms are still not known. It was investigated here, whether the bacterial lipopolysaccharides (LPS) might alter the tumor cell radiosensitivity. LPS were found to induce a radioresistance but solely in cells with an active TLR-4 pathway. Proteome profiling array revealed that LPS combined with irradiation resulted in a strong phosphorylation of cAMP response element-binding protein (CREB). Inhibition of CREB binding protein (CBP) by the specific inhibitor ICG-001 not only abrogated the LPS-induced radioresistance but even led to an increase in radiosensitivity. The sensitization caused by ICG-001 could be attributed to a reduction of DNA double-strand break (DSB) repair. It is shown that in NSCLC cells LPS leads to a CREB dependent radioresistance which is, however, reversible through CBP inhibition by the specific inhibitor ICG-001. These findings indicate that the combined treatment with radiation and CBP inhibition may improve survival of NSCLC patients suffering from pulmonary infections.

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