AAVvector-mediated in Vivo Reprogramming into Pluripotency

Overview

Journal Nat Commun

Specialty Biology

Date 2018 Jul 10

PMID 29985406

Citations 31

Authors

Elena Senis

Lluc Mosteiro

Stefan Wilkening

Ellen Wiedtke

Ali Nowrouzi

Saira Afzal

Raffaele Fronza

Henrik Landerer

Maria Abad

Dominik Niopek

Manfred Schmidt

Manuel Serrano

Dirk Grimm

Affiliations

Soon will be listed here.

Abstract

In vivo reprogramming of somatic cells into induced pluripotent stem cells (iPSC) holds vast potential for basic research and regenerative medicine. However, it remains hampered by a need for vectors to express reprogramming factors (Oct-3/4, Klf4, Sox2, c-Myc; OKSM) in selected organs. Here, we report OKSM delivery vectors based on pseudotyped Adeno-associated virus (AAV). Using the AAV-DJ capsid, we could robustly reprogram mouse embryonic fibroblasts with low vector doses. Swapping to AAV8 permitted to efficiently reprogram somatic cells in adult mice by intravenous vector delivery, evidenced by hepatic or extra-hepatic teratomas and iPSC in the blood. Notably, we accomplished full in vivo reprogramming without c-Myc. Most iPSC generated in vitro or in vivo showed transcriptionally silent, intronic or intergenic vector integration, likely reflecting the increased host genome accessibility during reprogramming. Our approach crucially advances in vivo reprogramming technology, and concurrently facilitates investigations into the mechanisms and consequences of AAV persistence.

Citing Articles

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