A Reprogrammable Mouse Strain from Gene-targeted Embryonic Stem Cells

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2009 Dec 17

PMID 20010832

Citations 118

Authors

Matthias Stadtfeld

Nimet Maherali

Marti Borkent

Konrad Hochedlinger

Affiliations

Soon will be listed here.

Abstract

The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming.

Citing Articles

An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.

Klagkou E, Valakos D, Foutadakis S, Polyzos A, Papadopoulou A, Vatsellas G Int J Mol Sci. 2024; 25(23).

PMID: 39684837 PMC: 11642860. DOI: 10.3390/ijms252313128.

Manipulating cell fate through reprogramming: approaches and applications.

Yagi M, Horng J, Hochedlinger K Development. 2024; 151(19).

PMID: 39348466 PMC: 11463964. DOI: 10.1242/dev.203090.

The interferon γ pathway enhances pluripotency and X-chromosome reactivation in iPSC reprogramming.

Barrero M, Lazarenkov A, Blanco E, Palma L, Lopez-Rubio A, Bauer M Sci Adv. 2024; 10(32):eadj8862.

PMID: 39110794 PMC: 11305397. DOI: 10.1126/sciadv.adj8862.

Retrospective identification of cell-intrinsic factors that mark pluripotency potential in rare somatic cells.

Jain N, Goyal Y, Dunagin M, Cote C, Mellis I, Emert B Cell Syst. 2024; 15(2):109-133.e10.

PMID: 38335955 PMC: 10940218. DOI: 10.1016/j.cels.2024.01.001.

Initiation phase cellular reprogramming ameliorates DNA damage in the ERCC1 mouse model of premature aging.

Paine P, Rechsteiner C, Morandini F, Desdin-Mico G, Mrabti C, Parras A Front Aging. 2024; 4():1323194.

PMID: 38322248 PMC: 10844398. DOI: 10.3389/fragi.2023.1323194.

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