TGF-β1 Negatively Regulates the Number and Function of Hematopoietic Stem Cells

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2018 Jun 26

PMID 29937145

Citations 28

Authors

Xiaofang Wang

Fang Dong

Sen Zhang

Wanzhu Yang

Wenying Yu

Zhao Wang

Shanshan Zhang

Jinhong Wang

Shihui Ma

Peng Wu

Yun Gao

Ji Dong

Fuchou Tang

Tao Cheng

Hideo Ema

Affiliations

Soon will be listed here.

Abstract

Transforming growth factor β1 (TGF-β1) plays a role in the maintenance of quiescent hematopoietic stem cells (HSCs) in vivo. We asked whether TGF-β1 controls the cell cycle status of HSCs in vitro to enhance the reconstitution activity. To examine the effect of TGF-β1 on the HSC function, we used an in vitro culture system in which single HSCs divide with the retention of their short- and long-term reconstitution ability. Extensive single-cell analyses showed that, regardless of its concentration, TGF-β1 slowed down the cell cycle progression of HSCs but consequently suppressed their self-renewal potential. Cycling HSCs were not able to go back to quiescence with TGF-β1. This study revealed a negative role of TGF-β1 in the regulation of the HSC number and reconstitution activity.

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