Specific Enzyme-linked Immunoassay for Rotavirus Serotypes 1 and 3

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1985 Aug 1

PMID 2993354

Citations 68

Authors

R D Shaw

M K Estes

H B Greenberg

Affiliations

Soon will be listed here.

Abstract

We prepared monoclonal antibodies against two serotypically distinct rotavirus strains: Wa, a serotype 1 virus of human origin, and rhesus rotavirus, a simian serotype 3 virus. Monoclonal antibodies which react specifically with VP7 of each serotype were identified by hemagglutination inhibition tests, plaque reduction neutralization studies, and solid-phase immunoassays which used wild-type and reassortant strains of rotavirus. An enzyme-linked immunoassay was designed which utilizes two of these antibodies to correctly identify serotype 1 and serotype 3 viruses.

Citing Articles

VP4- and VP7-specific antibodies mediate heterotypic immunity to rotavirus in humans.

Nair N, Feng N, Blum L, Sanyal M, Ding S, Jiang B Sci Transl Med. 2017; 9(395).

PMID: 28637924 PMC: 6312383. DOI: 10.1126/scitranslmed.aam5434.

The shift from low to high non-structural protein 1 expression in rotavirus-infected MA-104 cells.

Martinez-Alvarez L, Pina-Vazquez C, Zarco W, Padilla-Noriega L Mem Inst Oswaldo Cruz. 2013; 108(4):421-8.

PMID: 23827992 PMC: 3970611. DOI: 10.1590/S0074-0276108042013005.

Intestinal epithelia activate anti-viral signaling via intracellular sensing of rotavirus structural components.

Frias A, Vijay-Kumar M, Gentsch J, Crawford S, Carvalho F, Estes M Mucosal Immunol. 2010; 3(6):622-32.

PMID: 20664578 PMC: 2957552. DOI: 10.1038/mi.2010.39.

Development and validation of DNA microarray for genotyping group A rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) genes.

Honma S, Chizhikov V, Santos N, Tatsumi M, Timenetsky M, Linhares A J Clin Microbiol. 2007; 45(8):2641-8.

PMID: 17567783 PMC: 1951270. DOI: 10.1128/JCM.00736-07.

Rotavirus serotype G9 strains belonging to VP7 gene phylogenetic sequence lineage 1 may be more suitable for serotype G9 vaccine candidates than those belonging to lineage 2 or 3.

Hoshino Y, Jones R, Ross J, Honma S, Santos N, Gentsch J J Virol. 2004; 78(14):7795-802.

PMID: 15220453 PMC: 434108. DOI: 10.1128/JVI.78.14.7795-7802.2004.

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