Structure and Localization of Genes Encoding Aberrant and Normal Epidermal Growth Factor Receptor RNAs from A431 Human Carcinoma Cells

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1985 Jul 1

PMID 2991749

Citations 42

Authors

G T Merlino

S Ishii

J Whang-Peng

T Knutsen

Y H Xu

A J Clark

R H Stratton

R K Wilson

D P Ma

B A Roe

John Hunts

Nobuyoshi Shimizu

Ira Pastan

Affiliations

Soon will be listed here.

Abstract

A431 cells have an amplification of the epidermal growth factor (EGF) receptor gene, the cellular homolog of the v-erb B oncogene, and overproduce an aberrant 2.9-kilobase RNA that encodes a portion of the EGF receptor. A cDNA (pE15) for the aberrant RNA was cloned, sequenced, and used to analyze genomic DNA blots from A431 and normal cells. These data indicate that the aberrant RNA is created by a gene rearrangement within chromosome 7, resulting in a fusion of the 5' portion of the EGF receptor gene to an unidentified region of genomic DNA. The unidentified sequences are amplified to about the same degree (20- to 30-fold) as the EGF receptor sequences. In situ hybridization to chromosomes from normal cells and A431 cells show that both the EGF receptor gene and the unidentified DNA are localized to the p14-p12 region of chromosome 7. By using cDNA fragments to probe DNA blots from mouse-A431 somatic cell hybrids, the rearranged receptor gene was shown to be associated with translocation chromosome M4.

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References

Savage Jr C, Cohen S . Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization. J Biol Chem. 1972; 247(23):7609-11. View

Xu Y, Richert N, Ito S, Merlino G, Pastan I . Characterization of epidermal growth factor receptor gene expression in malignant and normal human cell lines. Proc Natl Acad Sci U S A. 1984; 81(23):7308-12. PMC: 392135. DOI: 10.1073/pnas.81.23.7308. View

Hollenberg M, Cuatrecasas P . Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin. Proc Natl Acad Sci U S A. 1973; 70(10):2964-8. PMC: 427148. DOI: 10.1073/pnas.70.10.2964. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Graf T, Royer-Pokora B, SCHUBERT G, Beug H . Evidence for the multiple oncogenic potential of cloned leukemia virus: in vitro and in vitro studies with avian erythroblastosis virus. Virology. 1976; 71(2):423-33. DOI: 10.1016/0042-6822(76)90370-6. View

Maniatis T, Kee S, Efstratiadis A, Kafatos F . Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976; 8(2):163-82. DOI: 10.1016/0092-8674(76)90001-5. View

Lembach K . Enhanced synthesis and extracellular accumulation of hyaluronic acid during stimulation of quiescent human fibroblasts by mouse epidermal growth factor. J Cell Physiol. 1976; 89(2):277-88. DOI: 10.1002/jcp.1040890211. View

Fabricant R, De Larco J, Todaro G . Nerve growth factor receptors on human melanoma cells in culture. Proc Natl Acad Sci U S A. 1977; 74(2):565-9. PMC: 392331. DOI: 10.1073/pnas.74.2.565. View

Das M, Miyakawa T, Fox C, Pruss R, Aharonov A, Herschman H . Specific radiolabeling of a cell surface receptor for epidermal growth factor. Proc Natl Acad Sci U S A. 1977; 74(7):2790-4. PMC: 431292. DOI: 10.1073/pnas.74.7.2790. View

10.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

11.

Sahyoun N, Hock R, Hollenberg M . Insulin and epidermal growth factor-urogastrone: affinity crosslinking to specific binding sites in rat liver membranes. Proc Natl Acad Sci U S A. 1978; 75(4):1675-9. PMC: 392401. DOI: 10.1073/pnas.75.4.1675. View

12.

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

13.

Shimizu N, Behzadian M, Shimizu Y . Genetics of cell surface receptors for bioactive polypeptides: binding of epidermal growth factor is associated with the presence of human chromosome 7 in human-mouse cell hybrids. Proc Natl Acad Sci U S A. 1980; 77(6):3600-4. PMC: 349665. DOI: 10.1073/pnas.77.6.3600. View

14.

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

15.

SANGER F, Coulson A, Barrell B, Smith A, Roe B . Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980; 143(2):161-78. DOI: 10.1016/0022-2836(80)90196-5. View

16.

Hayward W, Neel B, Astrin S . Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis. Nature. 1981; 290(5806):475-80. DOI: 10.1038/290475a0. View

17.

Hunter T, Cooper J . Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells. Cell. 1981; 24(3):741-52. DOI: 10.1016/0092-8674(81)90100-8. View

18.

Harper M, Saunders G . Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981; 83(3):431-9. DOI: 10.1007/BF00327364. View

19.

Cohen S, Ushiro H, Stoscheck C, Chinkers M . A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles. J Biol Chem. 1982; 257(3):1523-31. View

20.

Payne G, Bishop J, Varmus H . Multiple arrangements of viral DNA and an activated host oncogene in bursal lymphomas. Nature. 1982; 295(5846):209-14. DOI: 10.1038/295209a0. View