Nerve Growth Factor Receptors on Human Melanoma Cells in Culture

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Feb 1

PMID 265522

Citations 179

Authors

R N Fabricant

J E De Larco

G J Todaro

Affiliations

Soon will be listed here.

Abstract

Purified mouse nerve growth factor (NGF)radiolabeled with 125I was tested for its ability to bind to a variety of different cultured cells. NGF binds readily to human and hamster melanoma cells but does not bind to many other cell lines. The three cell lines with the highest number of NGF receptors were derived from metastatic melanomas. One of these lines, A875, was studied in detail and was shown to have approximately 7x10(5) NGF receptors per cell with an association constant of 1.0x10(9) liters/mole. The use of these cells in competition binding assays permits the detection of 0.25 ng of NGF in various biologic fluids. NGF can be shown to increase the survival, but not the division, of melanoma cells maintained in medium depleted of serum growth factors. This effect of NGF as a specific "survival factor" appears analogous to its effect on cultured sympathetic ganglion cells and on other cells derived from the neural crest.

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References

Moore G, LEHNER D, Kikuchi Y, LESS L . Continuous culture of a melanotic cell line from the golden hamster. Science. 1962; 137(3534):986-7. DOI: 10.1126/science.137.3534.986. View

GREENWOOD F, Hunter W, GLOVER J . THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963; 89:114-23. PMC: 1202279. DOI: 10.1042/bj0890114. View

Cohen S . Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962; 237:1555-62. View

Levi-Montalcini R, Meyer H, Hamburger V . In vitro experiments on the effects of mouse sarcomas 180 and 37 on the spinal and sympathetic ganglia of the chick embryo. Cancer Res. 1954; 14(1):49-57. View

Todaro G, De Larco J, Cohen S . Transformation by murine and feline sarcoma viruses specifically blocks binding of epidermal growth factor to cells. Nature. 1976; 264(5581):26-31. DOI: 10.1038/264026a0. View

Siggers D, Rogers J, Boyer S, Margolet L, Dorkin H, Banerjee S . Increased nerve-growth-factor beta-chain cross-reacting material in familial dysautonomia. N Engl J Med. 1976; 295(12):629-34. DOI: 10.1056/NEJM197609162951201. View

Revoltella R, Bertolini L, Pediconi M, Vigneti E . Specific binding of nerve growth factor (NGF) by murine C 1300 neuroblastoma cells. J Exp Med. 1974; 140(2):437-51. PMC: 2139591. DOI: 10.1084/jem.140.2.437. View

Oger J, Arnason B, Pantazis N, Lehrich J, Young M . Synthesis of nerve growth factor by L and 3T3 cells in culture. Proc Natl Acad Sci U S A. 1974; 71(4):1554-8. PMC: 388269. DOI: 10.1073/pnas.71.4.1554. View

Smith H, Scher C, Todaro G . Induction of cell division in medium lacking serum growth factor by SV40. Virology. 1971; 44(2):359-70. DOI: 10.1016/0042-6822(71)90267-4. View

10.

Hollenberg M, Cuatrecasas P . Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin. Proc Natl Acad Sci U S A. 1973; 70(10):2964-8. PMC: 427148. DOI: 10.1073/pnas.70.10.2964. View

11.

Armelin H . Pituitary extracts and steroid hormones in the control of 3T3 cell growth. Proc Natl Acad Sci U S A. 1973; 70(9):2702-6. PMC: 427087. DOI: 10.1073/pnas.70.9.2702. View

12.

Giard D, Aaronson S, Todaro G, ARNSTEIN P, Kersey J, Dosik H . In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst. 1973; 51(5):1417-23. DOI: 10.1093/jnci/51.5.1417. View

13.

Delarco J, Todaro G . Membrane receptors for murine leukemia viruses: characterization using the purified viral envelope glycoprotein, gp71. Cell. 1976; 8(3):365-71. DOI: 10.1016/0092-8674(76)90148-3. View

14.

Banerjee S, Snyder S, Cuatrecasas P, Greene L . Binding of nerve growth factor receptor in sympathetic ganglia. Proc Natl Acad Sci U S A. 1973; 70(9):2519-23. PMC: 427047. DOI: 10.1073/pnas.70.9.2519. View

15.

Rudland P, Seifert W, GOSPODAROWICZ D . Growth control in cultured mouse fibroblasts: induction of the pleiotypic and mitogenic responses by a purified growth factor. Proc Natl Acad Sci U S A. 1974; 71(7):2600-4. PMC: 388513. DOI: 10.1073/pnas.71.7.2600. View

16.

Levi-Montalcini R, ANGELETTI P . Nerve growth factor. Physiol Rev. 1968; 48(3):534-69. DOI: 10.1152/physrev.1968.48.3.534. View

17.

Cohen S, Carpenter G, Lembach K . Interaction of epidermal growth factor (EGF) with cultured fibroblasts. Adv Metab Disord. 1975; 8:265-84. DOI: 10.1016/b978-0-12-027308-9.50024-x. View

18.

Herrup K, SHOOTER E . Properties of the beta-nerve growth factor receptor in development. J Cell Biol. 1975; 67(1):118-25. PMC: 2109576. DOI: 10.1083/jcb.67.1.118. View

19.

Young M, Oger J, BLANCHARD M, Asdourian H, Amos H, Arnason B . Secretion of a nerve growth factor by primary chick fibroblast cultures. Science. 1975; 187(4174):361-2. DOI: 10.1126/science.1167427. View

20.

Varon S, Nomura J, SHOOTER E . The isolation of the mouse nerve growth factor protein in a high molecular weight form. Biochemistry. 1967; 6(7):2202-9. DOI: 10.1021/bi00859a043. View