F-Actin Nucleated on Chromosomes Coordinates Their Capture by Microtubules in Oocyte Meiosis

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2018 Jun 16

PMID 29903878

Citations 19

Authors

Mariia Burdyniuk

Andrea Callegari

Masashi Mori

Francois Nedelec

Peter Lenart

Affiliations

Soon will be listed here.

Abstract

Capture of each and every chromosome by spindle microtubules is essential to prevent chromosome loss and aneuploidy. In somatic cells, astral microtubules search and capture chromosomes forming lateral attachments to kinetochores. However, this mechanism alone is insufficient in large oocytes. We have previously shown that a contractile F-actin network is additionally required to collect chromosomes scattered in the 70-µm starfish oocyte nucleus. How this F-actin-driven mechanism is coordinated with microtubule capture remained unknown. Here, we show that after nuclear envelope breakdown Arp2/3-nucleated F-actin "patches" form around chromosomes in a Ran-GTP-dependent manner, and we propose that these structures sterically block kinetochore-microtubule attachments. Once F-actin-driven chromosome transport is complete, coordinated disassembly of F-actin patches allows synchronous capture by microtubules. Our observations indicate that this coordination is necessary because early capture of chromosomes by microtubules would interfere with F-actin-driven transport leading to chromosome loss and formation of aneuploid eggs.

Citing Articles

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