Characterization of Induced Pluripotent Stem Cell-derived Megakaryocyte Lysates for Potential Regenerative Applications

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2018 Jun 13

PMID 29893509

Citations 2

Authors

Anja Baigger

Dorothee Eicke

Yuliia Yuzefovych

Denys Pogozhykh

Rainer Blasczyk

Constanca Figueiredo

Affiliations

Soon will be listed here.

Abstract

Recently, platelet-derived growth factors present in lysates became an extreme interest in the field of regenerative medicine such as in wound healing and as substitutes to foetal bovine serum in xeno-free cell culture systems. However, the generation of such platelet lysates completely depends on the availability of platelet donors. In this study, the possibility to use in vitro-generated megakaryocytes derived from induced pluripotent stem cells (iPSCs) as a cell source for typical platelet growth factors was investigated. Therefore, the presence and levels of those factors were characterized in in vitro-produced megakaryocytes. In comparison with platelets, in vitro-generated megakaryocytes showed a multifold increased content in transcript and protein levels of typical platelet growth factors including platelet-derived growth factors (PDGFs), transforming growth factor (TGF)-1β, vascular endothelial cell factor (VEGF)-A, epidermal growth factor (EGF), insulin-like growth factor (IGF)-1 and tissue factor (TF). Hence, iPSC-derived megakaryocytes may serve as an efficient cell source for a donor-independent generation of growth factor-rich lysates with a broad application potential in innovative cell culture systems and regenerative therapies.

Citing Articles

Large-scale generation of megakaryocytes from human embryonic stem cells using transgene-free and stepwise defined suspension culture conditions.

Zhang B, Wu X, Zi G, He L, Wang S, Chen L Cell Prolif. 2021; 54(4):e13002.

PMID: 33615584 PMC: 8016648. DOI: 10.1111/cpr.13002.

Characterization of induced pluripotent stem cell-derived megakaryocyte lysates for potential regenerative applications.

Baigger A, Eicke D, Yuzefovych Y, Pogozhykh D, Blasczyk R, Figueiredo C J Cell Mol Med. 2018; 22(9):4545-4549.

PMID: 29893509 PMC: 6111809. DOI: 10.1111/jcmm.13698.

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