Elimination of Mycoplasma Contamination in Chlamydia Stocks As a Result of in Vivo Passage or Plaque Isolation

Overview

Journal BMC Res Notes

Publisher Biomed Central

Specialties Biology
General Medicine

Date 2018 Jun 9

PMID 29880030

Citations 1

Authors

Ira M Sigar

Justin H Schripsema

Kathleen A Kelly

Ashlesh K Murthy

Srikanth Manam

Kyle H Ramsey

Affiliations

Soon will be listed here.

Abstract

Objective: This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay.

Results: We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay.

Citing Articles

Mycoplasma decontamination in Chlamydia trachomatis culture: a curative approach.

Greer M, Elnaggar J, Taylor C, Shen L Pathog Dis. 2021; 79(9).

PMID: 34918079 PMC: 8759436. DOI: 10.1093/femspd/ftab056.

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