DNA Sequence of the Leftward Junction in the Adenovirus-simian Virus 40 Hybrid Ad2+D2 and Determination of the Structure of the D2-T Antigen

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1985 Jun 1

PMID 2987542

Citations 5

Authors

E A BAUMANN

C P Baur

M Baack

S Beck

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequence of the junction between the simian virus 40 early region and the adenovirus type 2 late region L4 in the hybrid virus Ad2+D2 was determined. The deduced amino acid sequence suggests that the D2-T antigen is a chimeric protein sharing 594 amino acids with the C-terminal end of the simian virus 40 T antigen and 104 amino acids with the N terminus of the adenovirus type 2 33,000-molecular-weight protein. The predicted structure of the D2-T antigen was confirmed by an immunoprecipitation analysis.

Citing Articles

An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase alpha and replicate SV40 DNA in vitro.

Weisshart K, Bradley M, Weiner B, Schneider C, Moarefi I, Fanning E J Virol. 1996; 70(6):3509-16.

PMID: 8648684 PMC: 190225. DOI: 10.1128/JVI.70.6.3509-3516.1996.

Database of mutations that alter the large tumor antigen in simian virus 40.

Kierstead T, Pipas J Nucleic Acids Res. 1996; 24(1):125-6.

PMID: 8594559 PMC: 145579. DOI: 10.1093/nar/24.1.125.

Effects of in vitro dephosphorylation on DNA-binding and DNA helicase activities of simian virus 40 large tumor antigen.

Klausing K, Scheidtmann K, BAUMANN E, Knippers R J Virol. 1988; 62(4):1258-65.

PMID: 2831386 PMC: 253135. DOI: 10.1128/JVI.62.4.1258-1265.1988.

Linker insertion mutants of simian virus 40 large T antigen that show trans-dominant interference with wild-type large T antigen map to multiple sites within the T-antigen gene.

Zhu J, Cole C J Virol. 1989; 63(11):4777-86.

PMID: 2552152 PMC: 251115. DOI: 10.1128/JVI.63.11.4777-4786.1989.

SV40 T antigen binds directly to the large subunit of purified DNA polymerase alpha.

Dornreiter I, Hoss A, Arthur A, Fanning E EMBO J. 1990; 9(10):3329-36.

PMID: 1698613 PMC: 552070. DOI: 10.1002/j.1460-2075.1990.tb07533.x.

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