Automated Analysis for Differentiating Leukocytes in Body Fluids Using the Software "biological Liquid Application" on ADVIA2120/2120i Hematology Analyzer

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2018 May 27

PMID 29802648

Citations 4

Authors

Alessandra Falda

Paolo Doretto

Affiliations

Soon will be listed here.

Abstract

Introduction: We evaluated the "Biological liquid application ADVIA2120" software for differentiating the percentage of polymorphonucleated (%PMN) and mononucleated cells (%MN) in ascitic, pleural, and peritoneal dialysis (PD) fluid.

Methods: Biological fluid test results of 193 specimens obtained by automated methods (87 with and 106 without dedicated software) were compared with May-Grünwald-Giemsa (MGG) stained blood smears. Limit of detection (LoD) and quantitation (LoQ), repeatability, and inaccuracy were assessed.

Results: Good agreement between the automated methods with dedicated software and the manual method for %PMN and %MN was obtained for leukocyte differentiation in ascitic and pleural fluids, while correlation with the manual method for PD fluid was poor, both with and without the dedicated software.

Conclusions: We demonstrated that the automated differentiation of leukocytes with dedicated software on the ADVIA2120 analyzer for body fluids is a good alternative to the microscopic reference method for peritoneal and pleural specimens, but not for PD fluids.

Citing Articles

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Automated cell count in body fluids: a review.

Alcaide Martin M, Altimira Queral L, Sahuquillo Frias L, Valina Amado L, Merino A, de Guadiana-Romualdo L Adv Lab Med. 2023; 2(2):149-177.

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Utilising low-cost, easy-to-use microscopy techniques for early peritonitis infection screening in peritoneal dialysis patients.

Buckup M, Kaneda J, Birk A, Glockner E, Venook R, Jain A Sci Rep. 2022; 12(1):14046.

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Automated analysis for differentiating leukocytes in body fluids using the software "biological liquid application" on ADVIA2120/2120i hematology analyzer.

Falda A, Doretto P J Clin Lab Anal. 2018; 32(8):e22578.

PMID: 29802648 PMC: 6817033. DOI: 10.1002/jcla.22578.

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