The Role of ScaRNAs in Adjusting Alternative MRNA Splicing in Heart Development

Overview

Journal J Cardiovasc Dev Dis

Specialty Cardiology & Vascular Diseases

Date 2018 May 9

PMID 29738469

Citations 16

Authors

Chloe Nagasawa

Allison Ogren

Nataliya Kibiryeva

Jennifer Marshall

James E OBrien

Naoya Kenmochi

Douglas C Bittel

Affiliations

Soon will be listed here.

Abstract

Congenital heart disease (CHD) is a leading cause of death in children <1 year of age. Despite intense effort in the last 10 years, most CHDs (~70%) still have an unknown etiology. Conotruncal based defects, such as Tetralogy of Fallot (TOF), a common complex of devastating heart defects, typically requires surgical intervention in the first year of life. We reported that the noncoding transcriptome in myocardial tissue from children with TOF is characterized by significant variation in levels of expression of noncoding RNAs, and more specifically, a significant reduction in 12 small cajal body-associated RNAs (scaRNAs) in the right ventricle. scaRNAs are essential for the biochemical modification and maturation of small nuclear RNAs (spliceosomal RNAs), which in turn are critical components of the spliceosome. This is particularly important because we also documented that splicing of mRNAs that are critical for heart development was dysregulated in the heart tissue of infants with TOF. Furthermore, we went on to show, using the zebrafish model, that altering the expression of these same scaRNAs led to faulty mRNA processing and heart defects in the developing embryo. This review will examine how scaRNAs may influence spliceosome fidelity in exon retention during heart development and thus contribute to regulation of heart development.

Citing Articles

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Small but strong: the emerging role of small nucleolar RNA in cardiovascular diseases.

Sun X, Wang G, Luo W, Gu H, Ma W, Wei X Front Cell Dev Biol. 2023; 11:1292925.

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Identification of mG regulator-mediated RNA methylation modification patterns and related immune microenvironment regulation characteristics in heart failure.

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