Mitochondria Export Sulfur Species Required for Cytosolic TRNA Thiolation

Overview

Journal Cell Chem Biol

Publisher Cell Press

Specialty Biochemistry

Date 2018 May 1

PMID 29706592

Citations 17

Authors

Alok Pandey

Jayashree Pain

Nathaniel Dziuba

Ashutosh K Pandey

Andrew Dancis

Paul A Lindahl

Debkumar Pain

Affiliations

Soon will be listed here.

Abstract

In eukaryotes, mitochondria have been hypothesized to generate sulfur species required for tRNA thiolation in the cytosol, although no direct evidence thus far exists. Here we have detected these sulfur species, making use of our observation that isolated yeast cytosol alone is unable to thiolate tRNAs but can do so upon addition of mitochondria. Mitochondria were found to utilize the cysteine desulfurase Nfs1 to produce sulfur-containing species with masses ranging from 700 to 1,100 Da. Mitochondria exported these species via the Atm1 transporter in the inner membrane. Once exported to the cytosol, these sulfur species promoted cytosolic tRNA thiolation with no further requirement of mitochondria. Furthermore, we found that the Isu1/2 scaffolds but not the Ssq1 chaperone of the mitochondrial iron-sulfur cluster machinery were required for cytosolic tRNA thiolation, and thus the sulfur utilization pathway bifurcates at the Isu1/2 site for intra-organellar use in mitochondria or export to the cytosol.

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