Characterization of Chickpea ( L.) Lectin for Biological Activity

Overview

Journal Physiol Mol Biol Plants

Specialty Biology

Date 2018 Apr 26

PMID 29692547

Citations 11

Authors

Ajay Kumar Gautam

Neha Gupta

Dakshita T Narvekar

Rajni Bhadkariya

Sameer S Bhagyawant

Affiliations

Soon will be listed here.

Abstract

Lectins are proteins that are subject of intense investigations. Information on lectin from chickpea ( L.) with respect to its biological activities are very limited. In this study, we purified lectin from the seeds of chickpea employing DEAE-cellulose and SP-Sephadex ion exchange chromatography and identified its molecular subunit mass as 35 kDa. The free radical scavenging activity of lectin measured by the DPPH assay has IC of 0.88 µg/mL. Lectin exerted antifungal activity against , , and , while antibacterial activity against , , and The minimum inhibitory concentrations were 200, 240, 160 and 140 µg for , and respectively. Lectin was further examined for its antiproliferative potential against cancerous cell line. The cell viability assay indicated a high inhibition activity on Ishikawa, HepG2, MCF-7 and MDA-MB-231 with IC value of 46.67, 44.20, 53.58 and 37.46 µg/mL respectively. These results can provide a background for future research into the benefits of chickpea lectin to pharmacological perspective.

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References

Chakraborti D, Sarkar A, Mondal H, Das S . Tissue specific expression of potent insecticidal, Allium sativum leaf agglutinin (ASAL) in important pulse crop, chickpea (Cicer arietinum L.) to resist the phloem feeding Aphis craccivora. Transgenic Res. 2009; 18(4):529-44. DOI: 10.1007/s11248-009-9242-7. View

Kiss R, Camby I, Duckworth C, De Decker R, Salmon I, Pasteels J . In vitro influence of Phaseolus vulgaris, Griffonia simplicifolia, concanavalin A, wheat germ, and peanut agglutinins on HCT-15, LoVo, and SW837 human colorectal cancer cell growth. Gut. 1997; 40(2):253-61. PMC: 1027058. DOI: 10.1136/gut.40.2.253. View

Luo Y, Xu X, Liu J, Li J, Sun Y, Liu Z . A novel mannose-binding tuber lectin from Typhonium divaricatum (L.) Decne (family Araceae) with antiviral activity against HSV-II and anti-proliferative effect on human cancer cell lines. J Biochem Mol Biol. 2007; 40(3):358-67. DOI: 10.5483/bmbrep.2007.40.3.358. View

Li Y, Zhang G, Ng T, Wang H . A novel lectin with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from dried fruiting bodies of the monkey head mushroom Hericium erinaceum. J Biomed Biotechnol. 2010; 2010:716515. PMC: 2896861. DOI: 10.1155/2010/716515. View

Ang A, Cheung R, Dan X, Chan Y, Pan W, Ng T . Purification and characterization of a glucosamine-binding antifungal lectin from Phaseolus vulgaris cv. Chinese pinto beans with antiproliferative activity towards nasopharyngeal carcinoma cells. Appl Biochem Biotechnol. 2013; 172(2):672-86. DOI: 10.1007/s12010-013-0542-2. View

Kumar S, Kapoor V, Gill K, Singh K, Xess I, Das S . Antifungal and antiproliferative protein from Cicer arietinum: a bioactive compound against emerging pathogens. Biomed Res Int. 2014; 2014:387203. PMC: 4053179. DOI: 10.1155/2014/387203. View

Ye X, Ng T . Isolation of a new cyclophilin-like protein from chickpeas with mitogenic, antifungal and anti-HIV-1 reverse transcriptase activities. Life Sci. 2002; 70(10):1129-38. DOI: 10.1016/s0024-3205(01)01473-4. View

Agrawal P, Kumar S, Jaiswal Y, Das H, Das R . A Mesorhizobium lipopolysaccharide (LPS) specific lectin (CRL) from the roots of nodulating host plant, Cicer arietinum. Biochimie. 2010; 93(3):440-9. DOI: 10.1016/j.biochi.2010.10.017. View

Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D . New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990; 82(13):1107-12. DOI: 10.1093/jnci/82.13.1107. View

10.

Xia L, Ng T . A hemagglutinin with mitogenic activity from dark red kidney beans. J Chromatogr B Analyt Technol Biomed Life Sci. 2006; 844(2):213-6. DOI: 10.1016/j.jchromb.2006.07.042. View

11.

Wakankar M, Krishnasastry M, Jaokar T, Patel K, Gaikwad S . Solution and in silico studies on the recombinant lectin from Cicer arietinum seeds. Int J Biol Macromol. 2013; 56:149-55. DOI: 10.1016/j.ijbiomac.2013.02.015. View

12.

Manach C, Scalbert A, Morand C, Remesy C, Jimenez L . Polyphenols: food sources and bioavailability. Am J Clin Nutr. 2004; 79(5):727-47. DOI: 10.1093/ajcn/79.5.727. View

13.

Bourne Y, Ayouba A, Rouge P, Cambillau C . Interaction of a legume lectin with two components of the bacterial cell wall. A crystallographic study. J Biol Chem. 1994; 269(13):9429-35. View

14.

Faheina-Martins G, da Silveira A, Cavalcanti B, Ramos M, Moraes M, Pessoa C . Antiproliferative effects of lectins from Canavalia ensiformis and Canavalia brasiliensis in human leukemia cell lines. Toxicol In Vitro. 2012; 26(7):1161-9. DOI: 10.1016/j.tiv.2012.06.017. View

15.

Gomes F, Procopio T, Napoleao T, Coelho L, Paiva P . Antimicrobial lectin from Schinus terebinthifolius leaf. J Appl Microbiol. 2012; 114(3):672-9. DOI: 10.1111/jam.12086. View

16.

Kaur N, Dhuna V, Kamboj S, Agrewala J, Singh J . A novel antiproliferative and antifungal lectin from Amaranthus viridis Linn seeds. Protein Pept Lett. 2006; 13(9):897-905. DOI: 10.2174/092986606778256153. View

17.

Lin P, Ye X, Ng T . Purification of melibiose-binding lectins from two cultivars of Chinese black soybeans. Acta Biochim Biophys Sin (Shanghai). 2008; 40(12):1029-38. PMC: 7110201. DOI: 10.1111/j.1745-7270.2008.00488.x. View

18.

Llorach R, Espin J, Tomas-Barberan F, Ferreres F . Valorization of cauliflower (Brassica oleracea L. var. botrytis) by-products as a source of antioxidant phenolics. J Agric Food Chem. 2003; 51(8):2181-7. DOI: 10.1021/jf021056a. View

19.

Baker R, Brown R, Chen Z, Cleveland T, Fakhoury A . A maize lectin-like protein with antifungal activity against Aspergillus flavus. J Food Prot. 2009; 72(1):120-7. DOI: 10.4315/0362-028x-72.1.120. View

20.

Vogelsang R, Barz W . Purification, characterization and differential hormonal regulation of a beta-1,3-glucanase and two chitinases from chickpea (Cicer arietinum L.). Planta. 1993; 189(1):60-9. DOI: 10.1007/BF00201344. View