BAP1 Induces Cell Death Via Interaction with 14-3-3 in Neuroblastoma

Overview

Journal Cell Death Dis

Specialties Cell Biology
General Medicine

Date 2018 Apr 25

PMID 29686263

Citations 22

Authors

Wondossen Sime

Qiankun Niu

Yasmin Abassi

Katarzyna Chmielarska Masoumi

Reihaneh Zarrizi

Julie Bonne Kohler

Sven Kjellstrom

Vito Alessandro Lasorsa

Mario Capasso

Haian Fu

Ramin Massoumi

Affiliations

Soon will be listed here.

Abstract

BRCA1-associated protein 1 (BAP1) is a nuclear deubiquitinating enzyme that is associated with multiprotein complexes that regulate key cellular pathways, including cell cycle, cellular differentiation, cell death, and the DNA damage response. In this study, we found that the reduced expression of BAP1 pro6motes the survival of neuroblastoma cells, and restoring the levels of BAP1 in these cells facilitated a delay in S and G2/M phase of the cell cycle, as well as cell apoptosis. The mechanism that BAP1 induces cell death is mediated via an interaction with 14-3-3 protein. The association between BAP1 and 14-3-3 protein releases the apoptotic inducer protein Bax from 14-3-3 and promotes cell death through the intrinsic apoptosis pathway. Xenograft studies confirmed that the expression of BAP1 reduces tumor growth and progression in vivo by lowering the levels of pro-survival factors such as Bcl-2, which in turn diminish the survival potential of the tumor cells. Patient data analyses confirmed the finding that the high-BAP1 mRNA expression correlates with a better clinical outcome. In summary, our study uncovers a new mechanism for BAP1 in the regulation of cell apoptosis in neuroblastoma cells.

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