Hybrid Sterility in Fish Caused by Mitotic Arrest of Primordial Germ Cells

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2018 Apr 4

PMID 29610216

Citations 15

Authors

Hiroyuki Yoshikawa

Dongdong Xu

Yasuko Ino

Tasuku Yoshino

Takao Hayashida

Junjie Wang

Ryosuke Yazawa

Goro Yoshizaki

Yutaka Takeuchi

Affiliations

Soon will be listed here.

Abstract

Sterility in hybrid animals is widely known to be due to a cytological mechanism of aberrant homologous chromosome pairing during meiosis in hybrid germ cells. In this study, the gametes of four marine fish species belonging to the Sciaenid family were artificially fertilized, and germ cell development was examined at the cellular and molecular levels. One of the intergeneric hybrids had gonads that were testis-like in structure, small in size, and lacked germ cells. Specification of primordial germ cells (PGCs) and their migration toward genital ridges occurred normally in hybrid embryos, but these PGCs did not proliferate in the hybrid gonads. By germ cell transplantation assay, we showed that the gonadal microenvironment in hybrid recipients produced functional donor-derived gametes, suggesting that the germ cell-less phenotype was caused by cell autonomous proliferative defects of hybrid PGCs. This is the first evidence of mitotic arrest of germ cells causing hybrid sterility in animals.

Citing Articles

Embryonic Lethality, Juvenile Growth Variation, and Adult Sterility Correlate With Phylogenetic Distance of Danionin Hybrids.

Trevena R, Veire B, Chamberlain T, Moravec C, Pelegri F Evol Dev. 2024; 27(1):e12495.

PMID: 39639649 PMC: 11621593. DOI: 10.1111/ede.12495.

Integrated miRNA and mRNA Sequencing Reveals the Sterility Mechanism in Hybrid Yellow Catfish Resulting from (♀) × (♂).

Li S, Yang Q, Li M, Lan Y, Song Z Animals (Basel). 2024; 14(11).

PMID: 38891632 PMC: 11171309. DOI: 10.3390/ani14111586.

Genome-wide comparative methylation analysis reveals the fate of germ stem cells after surrogate production in teleost.

Nayak R, Franek R, Laurent A, Psenicka M BMC Biol. 2024; 22(1):39.

PMID: 38360607 PMC: 10870548. DOI: 10.1186/s12915-024-01842-z.

Genetic and karyotype divergence between parents affect clonality and sterility in hybrids.

Marta A, Tichopad T, Bartos O, Klima J, Shah M, Bohlen V Elife. 2023; 12.

PMID: 37930936 PMC: 10627513. DOI: 10.7554/eLife.88366.

Production of all-male non-transgenic zebrafish by conditional primordial germ cell ablation.

Wang F, Feng Y, Wang X, Ou M, Zhang X, Zhao J Fish Physiol Biochem. 2023; 49(6):1215-1227.

PMID: 37857788 DOI: 10.1007/s10695-023-01252-y.

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