Analysis on the Meiosis-Related Gene (Dmc1, Ph1) Expression in Autotriploid Carassius Auratus

Overview

Journal Mar Biotechnol (NY)

Specialties Biology
Biotechnology

Date 2019 Sep 15

PMID 31520246

Citations 4

Authors

Qinbo Qin

Yuwei Zhou

Chongqing Wang

Minghe Zhang

Huan Qin

Chun Zhao

Shaojun Liu

Affiliations

Soon will be listed here.

Abstract

Triploid is usually considered to be unable to perform normal meiosis due to the abnormal behavior of the three sets of chromosomes. But autotriploid Carassius auratus in the Dongting water system (3n = 150, abbreviated as 3nCC) can perform normal meiosis. In artificial autotriploid Carassius auratus (3n = 150, abbreviated as 3nRR), female individuals undergo normal meiosis and produce mature gametes, while male individuals cannot. To better understand the effects of triploidization on meiosis in fish, we study the structure, methylation level, and expression level of meiosis-related genes (Dmc1, Ph1) in diploid Carassius auratus (2n = 100, abbreviated as 2nCC), Carassius auratus red var.(2n = 100, abbreviated as RCC), 3nCC and 3nRR. The results show that, compared with their diploid ancestors (2nCC and RCC), Dmc1 and Ph1 genes are hypomethylated in all 3nCC and female 3nRR, while are hypermethylated in male 3nRR. Correspondingly, Dmc1 and Ph1 genes are highly expressed in all 3nCC and female 3nRR, while are lowly expressed in male 3nRR. These results indicate that high expression of meiosis-related genes can contribute to restoration of bivalent pairing during meiosis in autotriploid Carassius auratus. This study provides new insights into the effect of DNA methylation on the fertility in triploid fish.

Citing Articles

Expression and localization of HPG axis-related genes in Carassius auratus with different ploidy.

Xu X, Yang L, Deng X, Xiao Q, Huang X, Wang C Front Endocrinol (Lausanne). 2024; 15:1336679.

PMID: 38410696 PMC: 10894961. DOI: 10.3389/fendo.2024.1336679.

Comparative transcriptomic analysis of reproductive characteristics of reciprocal hybrid lineages derived from hybridization between Megalobrama amblycephala and Culter alburnus.

Ding X, Zhang Y, Li D, Xu J, Wu C, Cui X BMC Genom Data. 2023; 24(1):45.

PMID: 37573319 PMC: 10422732. DOI: 10.1186/s12863-023-01141-6.

The alternative transcription and expression characterization of in autotetraploid .

Xu X, Wang C, Xiao Q, Huang X, Zhou Y, Luo X Front Genet. 2023; 14:1135006.

PMID: 37056290 PMC: 10086133. DOI: 10.3389/fgene.2023.1135006.

Formation of autotriploid Carassius auratus and its fertility-related genes analysis.

Wang C, Luo X, Qin H, Zhao C, Yang L, Yu T BMC Genomics. 2021; 22(1):435.

PMID: 34107878 PMC: 8191051. DOI: 10.1186/s12864-021-07753-5.

References

Boden S, Langridge P, Spangenberg G, Able J . TaASY1 promotes homologous chromosome interactions and is affected by deletion of Ph1. Plant J. 2008; 57(3):487-97. DOI: 10.1111/j.1365-313X.2008.03701.x. View

Liu B, Wendel J . Epigenetic phenomena and the evolution of plant allopolyploids. Mol Phylogenet Evol. 2003; 29(3):365-79. DOI: 10.1016/s1055-7903(03)00213-6. View

He W, Qin Q, Liu S, Li T, Wang J, Xiao J . Organization and variation analysis of 5S rDNA in different ploidy-level hybrids of red crucian carp × topmouth culter. PLoS One. 2012; 7(6):e38976. PMC: 3377697. DOI: 10.1371/journal.pone.0038976. View

Comai L . The advantages and disadvantages of being polyploid. Nat Rev Genet. 2005; 6(11):836-46. DOI: 10.1038/nrg1711. View

Ma X, Gustafson J . Genome evolution of allopolyploids: a process of cytological and genetic diploidization. Cytogenet Genome Res. 2005; 109(1-3):236-49. DOI: 10.1159/000082406. View

Wei L, Xu F, Wang Y, Cai Z, Yu W, He C . The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas. Mar Biotechnol (NY). 2018; 20(4):425-435. DOI: 10.1007/s10126-018-9806-8. View

Qin Q, Cao L, Wang Y, Ren L, Liu Q, Zhou Y . Rapid Genomic and Genetic Changes in the First Generation of Autotetraploid Lineages Derived from Distant Hybridization of Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂). Mar Biotechnol (NY). 2018; 21(2):139-149. PMC: 6441405. DOI: 10.1007/s10126-018-9859-8. View

Jaillon O, Aury J, Brunet F, Petit J, Stange-Thomann N, Mauceli E . Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype. Nature. 2004; 431(7011):946-57. DOI: 10.1038/nature03025. View

Bishop J, Kim S, Villamil M, Lee D, Rayburn A . Meiotic pairing as an indicator of genome composition in polyploid prairie cordgrass (Spartina pectinata Link). Genetica. 2017; 145(2):235-240. DOI: 10.1007/s10709-017-9955-0. View

10.

He W, Tu C, Liu Q, Meng L, Yuan S, Luo A . mutation that causes human non-obstructive azoospermia and premature ovarian insufficiency identified by whole-exome sequencing. J Med Genet. 2018; 55(3):198-204. DOI: 10.1136/jmedgenet-2017-104992. View

11.

Vandepoele K, De Vos W, Taylor J, Meyer A, Van de Peer Y . Major events in the genome evolution of vertebrates: paranome age and size differ considerably between ray-finned fishes and land vertebrates. Proc Natl Acad Sci U S A. 2004; 101(6):1638-43. PMC: 341801. DOI: 10.1073/pnas.0307968100. View

12.

Yoshikawa H, Xu D, Ino Y, Yoshino T, Hayashida T, Wang J . Hybrid Sterility in Fish Caused by Mitotic Arrest of Primordial Germ Cells. Genetics. 2018; 209(2):507-521. PMC: 5972423. DOI: 10.1534/genetics.118.300777. View

13.

Qin Q, Wang Y, Wang J, Dai J, Xiao J, Hu F . The autotetraploid fish derived from hybridization of Carassius auratus red var. (female) × Megalobrama amblycephala (male). Biol Reprod. 2014; 91(4):93. DOI: 10.1095/biolreprod.114.122283. View

14.

Liu S, Qin Q, Xiao J, Lu W, Shen J, Li W . The formation of the polyploid hybrids from different subfamily fish crossings and its evolutionary significance. Genetics. 2007; 176(2):1023-34. PMC: 1894572. DOI: 10.1534/genetics.107.071373. View

15.

Qin Q, Wang J, Hu M, Huang S, Liu S . Autotriploid origin of Carassius auratus as revealed by chromosomal locus analysis. Sci China Life Sci. 2016; 59(6):622-6. DOI: 10.1007/s11427-016-5040-7. View

16.

Bird K, VanBuren R, Puzey J, Edger P . The causes and consequences of subgenome dominance in hybrids and recent polyploids. New Phytol. 2018; 220(1):87-93. DOI: 10.1111/nph.15256. View

17.

Xiao J, Zou T, Chen Y, Chen L, Liu S, Tao M . Coexistence of diploid, triploid and tetraploid crucian carp (Carassius auratus) in natural waters. BMC Genet. 2011; 12:20. PMC: 3040159. DOI: 10.1186/1471-2156-12-20. View

18.

Chen Z, Pikaard C . Transcriptional analysis of nucleolar dominance in polyploid plants: biased expression/silencing of progenitor rRNA genes is developmentally regulated in Brassica. Proc Natl Acad Sci U S A. 1997; 94(7):3442-7. PMC: 20389. DOI: 10.1073/pnas.94.7.3442. View

19.

Cifuentes M, Rivard M, Pereira L, Chelysheva L, Mercier R . Haploid meiosis in Arabidopsis: double-strand breaks are formed and repaired but without synapsis and crossovers. PLoS One. 2013; 8(8):e72431. PMC: 3737152. DOI: 10.1371/journal.pone.0072431. View

20.

Piferrer F, Ribas L, Diaz N . Genomic approaches to study genetic and environmental influences on fish sex determination and differentiation. Mar Biotechnol (NY). 2012; 14(5):591-604. PMC: 3419836. DOI: 10.1007/s10126-012-9445-4. View