Determination of the Subcellular Localization and Mechanism of Action of Ferrostatins in Suppressing Ferroptosis

Overview

Journal ACS Chem Biol

Publisher American Chemical Society

Specialties Biochemistry
Biology

Date 2018 Mar 8

PMID 29512999

Citations 165

Authors

Michael M Gaschler

Fanghao Hu

Huizhong Feng

Andreas Linkermann

Wei Min

Brent R Stockwell

Affiliations

Soon will be listed here.

Abstract

Ferroptosis is a form of nonapoptotic cell death characterized by the unchecked accumulation of lipid peroxides. Ferrostatin-1 and its analogs (ferrostatins) specifically prevent ferroptosis in multiple contexts, but many aspects of their molecular mechanism of action remain poorly described. Here, we employed stimulated Raman scattering (SRS) microscopy coupled with small vibrational tags to image the distribution of ferrostatins in cells and found that they accumulate in lysosomes, mitochondria, and the endoplasmic reticulum. We then evaluated the functional relevance of lysosomes and mitochondria to ferroptosis suppression by ferrostatins and found that neither is required for effective ferroptosis suppression.

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