CAMPr: A Single-wavelength Fluorescent Sensor for Cyclic AMP

Overview

Journal Sci Signal

Specialties Cell Biology
Physiology
Science

Date 2018 Mar 8

PMID 29511120

Citations 36

Authors

Christopher R Hackley

Esteban O Mazzoni

Justin Blau

Affiliations

Soon will be listed here.

Abstract

Genetically encoded fluorescent sensors enable cell-specific measurements of ions and small molecules in real time. Cyclic adenosine monophosphate (cAMP) is one of the most important signaling molecules in virtually all cell types and organisms. We describe cAMPr, a new single-wavelength cAMP sensor. We developed cAMPr in bacteria and embryonic stem cells and validated the sensor in mammalian neurons in vitro and in circadian pacemaker neurons in intact brains. Comparison with other single-wavelength cAMP sensors showed that cAMPr improved the quantitative detection of cAMP abundance. In addition, cAMPr is compatible with both single-photon and two-photon imaging. This enabled us to use cAMPr together with the red fluorescent Ca sensor RCaMP1h to simultaneously monitor Ca and cAMP in brains. Thus, cAMPr is a new and versatile genetically encoded cAMP sensor.

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