Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light Up Cellular Signals

Overview

Journal Chem Rev

Publisher American Chemical Society

Specialty Chemistry

Date 2024 Nov 13

PMID 39535501

Authors

Anneliese M M Gest

Ayse Z Sahan

Yanghao Zhong

Wei Lin

Sohum Mehta

Jin Zhang

Affiliations

Soon will be listed here.

Abstract

Cellular function is controlled through intricate networks of signals, which lead to the myriad pathways governing cell fate. Fluorescent biosensors have enabled the study of these signaling pathways in living systems across temporal and spatial scales. Over the years there has been an explosion in the number of fluorescent biosensors, as they have become available for numerous targets, utilized across spectral space, and suited for various imaging techniques. To guide users through this extensive biosensor landscape, we discuss critical aspects of fluorescent proteins for consideration in biosensor development, smart tagging strategies, and the historical and recent biosensors of various types, grouped by target, and with a focus on the design and recent applications of these sensors in living systems.

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