Skeletal Muscle Stem Cells Adopt a Dormant Cell State Post Mortem and Retain Regenerative Capacity

Overview

Journal Nat Commun

Specialty Biology

Date 2012 Jun 14

PMID 22692546

Citations 70

Authors

Mathilde Latil

Pierre Rocheteau

Laurent Chatre

Serena Sanulli

Sylvie Memet

Miria Ricchetti

Shahragim Tajbakhsh

Fabrice Chretien

Affiliations

Soon will be listed here.

Abstract

The accessibility to stem cells from healthy or diseased individuals, and the maintenance of their potency are challenging issues for stem cell biology. Here we report the isolation of viable and functional skeletal myogenic cells from humans up to 17 days, and mice up to 14 days post mortem, much longer beyond previous reports. Muscle stem cells are enriched in post mortem tissue, suggesting a selective survival advantage compared with other cell types. Transplantation of mouse muscle and haematopoietic stem cells regenerates tissues robustly. Cellular quiescence contributes to this cell viability where cells adopt a reversible dormant state characterized by reduced metabolic activity, a prolonged lag phase before the first cell division, elevated levels of reactive oxygen species and a transcriptional status less primed for commitment. Finally, severe hypoxia, or anoxia is critical for maintaining stem cell viability and regenerative capacity. Thus, these cells provide a useful resource for studying stem cell biology.

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