SLAMF1 is Required for TLR4-mediated TRAM-TRIF-dependent Signaling in Human Macrophages

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2018 Feb 15

PMID 29440514

Citations 26

Authors

Maria Yurchenko

Astrid Skjesol

Liv Ryan

Gabriel Mary Richard

Richard Kumaran Kandasamy

Ninghai Wang

Cox Terhorst

Harald Husebye

Terje Espevik

Affiliations

Soon will be listed here.

Abstract

Signaling lymphocytic activation molecule family 1 (SLAMF1) is an Ig-like receptor and a costimulatory molecule that initiates signal transduction networks in a variety of immune cells. In this study, we report that SLAMF1 is required for Toll-like receptor 4 (TLR4)-mediated induction of interferon β (IFNβ) and for killing of Gram-negative bacteria by human macrophages. We found that SLAMF1 controls trafficking of the Toll receptor-associated molecule (TRAM) from the endocytic recycling compartment (ERC) to phagosomes. In resting macrophages, SLAMF1 is localized to ERC, but upon addition of , it is trafficked together with TRAM from ERC to phagosomes in a Rab11-dependent manner. We found that endogenous SLAMF1 protein interacted with TRAM and defined key interaction domains as amino acids 68 to 95 of TRAM as well as 15 C-terminal amino acids of SLAMF1. Interestingly, the SLAMF1-TRAM interaction was observed for human but not mouse proteins. Overall, our observations suggest that SLAMF1 is a new target for modulation of TLR4-TRAM-TRIF inflammatory signaling in human cells.

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