Reduction of Hexavalent Chromium and Detection of Chromate Reductase (ChrR) in Stenotrophomonas Maltophilia

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2018 Feb 14

PMID 29438314

Citations 17

Authors

Rosa Baldiris

Natali Acosta-Tapia

Alfredo Montes

Jennifer Hernandez

Ricardo Vivas-Reyes

Affiliations

Soon will be listed here.

Abstract

An Gram negative strain of , indigenous to environments contaminated by Cr(VI) and identified by biochemical methods and 16S rRNA gene analysis, reduced chromate by 100%, 98-99% and 92% at concentrations in the 10-70, 80-300, and 500 mg/L range, respectively at pH 7 and temperature 37 °C. Increasing concentrations of Cr(VI) in the medium lowered the growth rate but could not be directly correlated with the amount of Cr(VI) reduced. The strain also exhibited multiple resistance to antibiotics and tolerance and resistance to various heavy metals (Ni, Zn and Cu), with the exception of Hg. Hexavalent chromium reduction was mainly associated with the soluble fraction of the cell evaluated with crude cell-free extracts. A protein of molecular weight around 25 kDa was detected on SDS-PAGE gel depending on the concentration of hexavalent chromium in the medium (0, 100 and 500 mg/L). In silico analysis in this contribution, revealed the presence of the chromate reductase gene in , evidenced through a fragment of around 468 bp obtained experimentally. High Cr(VI) concentration resistance and high Cr(VI) reducing ability of the strain make it a suitable candidate for bioremediation.

Citing Articles

Efficacy of Lacticaseibacillus rhamnosus probiotic strains in treating chromate induced dermatitis.

Ali M, Amin A, Abbas Z Sci Rep. 2025; 15(1):8796.

PMID: 40087372 DOI: 10.1038/s41598-025-93732-9.

Characterization and Molecular Insights of a Chromium-Reducing Bacterium .

Tuli S, Ali M, Jamal T, Khan M, Fatima N, Ahmed I Microorganisms. 2025; 12(12.

PMID: 39770835 PMC: 11676387. DOI: 10.3390/microorganisms12122633.

Physicochemistry and comparative metagenomics of a tropical estuary persistently inundated with anthropogenic pollutants.

Salam L, Apollos E, Obayori O, Michael G Folia Microbiol (Praha). 2024; .

PMID: 39621289 DOI: 10.1007/s12223-024-01227-3.

Unveiling the chromate stress response in the marine bacterium Bacillus enclensis AGM_Cr8: a multifaceted investigation.

Monga A, Fulke A, Sonker S, Dasgupta D World J Microbiol Biotechnol. 2024; 40(12):394.

PMID: 39586856 DOI: 10.1007/s11274-024-04206-5.

An animal charcoal contaminated cottage industry soil highlighted by halophilic archaea dominance and decimation of bacteria.

Obayori O, Salam L, Ashade A, Oseni T, Kalu M, Mustapha F World J Microbiol Biotechnol. 2024; 40(10):327.

PMID: 39299940 DOI: 10.1007/s11274-024-04136-2.

References

Zahoor A, Rehman A . Isolation of Cr(VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater. J Environ Sci (China). 2009; 21(6):814-20. DOI: 10.1016/s1001-0742(08)62346-3. View

Gupta V, Rastogi A . Biosorption of hexavalent chromium by raw and acid-treated green alga Oedogonium hatei from aqueous solutions. J Hazard Mater. 2008; 163(1):396-402. DOI: 10.1016/j.jhazmat.2008.06.104. View

Ramirez-Diaz M, Diaz-Perez C, Vargas E, Riveros-Rosas H, Campos-Garcia J, Cervantes C . Mechanisms of bacterial resistance to chromium compounds. Biometals. 2007; 21(3):321-32. DOI: 10.1007/s10534-007-9121-8. View

Gadd G . Metals, minerals and microbes: geomicrobiology and bioremediation. Microbiology (Reading). 2009; 156(Pt 3):609-643. DOI: 10.1099/mic.0.037143-0. View

Magiorakos A, Srinivasan A, Carey R, Carmeli Y, Falagas M, Giske C . Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2011; 18(3):268-81. DOI: 10.1111/j.1469-0691.2011.03570.x. View

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-9. PMC: 3203626. DOI: 10.1093/molbev/msr121. View

Ge H, Ma Z . Microwave preparation of triethylenetetramine modified graphene oxide/chitosan composite for adsorption of Cr(VI). Carbohydr Polym. 2015; 131:280-7. DOI: 10.1016/j.carbpol.2015.06.025. View

Ge S, Ge S . Simultaneous Cr(VI) reduction and Zn(II) biosorption by Stenotrophomonas sp. and constitutive expression of related genes. Biotechnol Lett. 2016; 38(5):877-84. DOI: 10.1007/s10529-016-2057-8. View

Oves M, Khan M, Zaidi A, Ahmed A, Ahmed F, Ahmad E . Antibacterial and cytotoxic efficacy of extracellular silver nanoparticles biofabricated from chromium reducing novel OS4 strain of Stenotrophomonas maltophilia. PLoS One. 2013; 8(3):e59140. PMC: 3605433. DOI: 10.1371/journal.pone.0059140. View

10.

Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I . Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. 2007; 115(8):891-9. DOI: 10.1111/j.1600-0463.2007.apm_630.x. View

11.

Pages J, James C, Winterhalter M . The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria. Nat Rev Microbiol. 2008; 6(12):893-903. DOI: 10.1038/nrmicro1994. View

12.

Chai L, Huang S, Yang Z, Peng B, Huang Y, Chen Y . Cr (VI) remediation by indigenous bacteria in soils contaminated by chromium-containing slag. J Hazard Mater. 2009; 167(1-3):516-22. DOI: 10.1016/j.jhazmat.2009.01.030. View

13.

Waters V, Atenafu E, Lu A, Yau Y, Tullis E, Ratjen F . Chronic Stenotrophomonas maltophilia infection and mortality or lung transplantation in cystic fibrosis patients. J Cyst Fibros. 2013; 12(5):482-6. DOI: 10.1016/j.jcf.2012.12.006. View

14.

Palleroni N, BRADBURY J . Stenotrophomonas, a new bacterial genus for Xanthomonas maltophilia (Hugh 1980) Swings et al. 1983. Int J Syst Bacteriol. 1993; 43(3):606-9. DOI: 10.1099/00207713-43-3-606. View

15.

Kumari V, Yadav A, Haq I, Kumar S, Bharagava R, Singh S . Genotoxicity evaluation of tannery effluent treated with newly isolated hexavalent chromium reducing Bacillus cereus. J Environ Manage. 2016; 183:204-211. DOI: 10.1016/j.jenvman.2016.08.017. View

16.

Kummerer K . Resistance in the environment. J Antimicrob Chemother. 2004; 54(2):311-20. DOI: 10.1093/jac/dkh325. View

17.

OToole G, Kolter R . Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol. 1998; 28(3):449-61. DOI: 10.1046/j.1365-2958.1998.00797.x. View

18.

Quintelas C, Fonseca B, Silva B, Figueiredo H, Tavares T . Treatment of chromium(VI) solutions in a pilot-scale bioreactor through a biofilm of Arthrobacter viscosus supported on GAC. Bioresour Technol. 2008; 100(1):220-6. DOI: 10.1016/j.biortech.2008.05.010. View

19.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

20.

Ahemad M . Enhancing phytoremediation of chromium-stressed soils through plant-growth-promoting bacteria. J Genet Eng Biotechnol. 2019; 13(1):51-58. PMC: 6299803. DOI: 10.1016/j.jgeb.2015.02.001. View