Biotransformation of Chromium (VI) Via a Reductant Activity from the Fungal Strain

Overview

Journal J Fungi (Basel)

Date 2021 Dec 24

PMID 34947004

Authors

Juan Fernando Cardenas Gonzalez

Ismael Acosta Rodriguez

Yolanda Teran Figueroa

Patricia Lappe Oliveras

Rebeca Martinez Flores

Adriana Sarai Rodriguez Perez

Affiliations

Soon will be listed here.

Abstract

Industrial effluents from chromium-based products lead to chromium pollution in the environment. Several technologies have been employed for the removal of chromium (Cr) from the environment, including adsorption, ion-exchange, bioremediation, etc. In this study, we isolated a Cr (VI)-resistant fungus, from contaminated soil, which could reduce chromium. We also characterized a reductant activity of dichromate found in the cellular fraction of the fungus: optimal pH and temperature, effect of enzymatic inhibitors and enhancers, metal ions, use of electron donors, and initial Cr (VI) and protein concentration. This study also shows possible mechanisms that could be involved in the elimination of this metal. We observed an increase in the reduction of Cr (VI) activity in the presence of NADH followed by that of formate and acetate, as electron donor. This reduction was highly inhibited by EDTA followed by NaN and KCN, and this activity showed the highest activity at an optimal pH of 7.0 at 37 °C with a protein concentration of 3.62 µg/mL.

References

Lee K, Buckley H, CAMPBELL C . An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans. Sabouraudia. 1975; 13(2):148-53. DOI: 10.1080/00362177585190271. View

Bahafid W, Tahri Joutey N, Sayel H, Boularab I, El Ghachtouli N . Bioaugmentation of chromium-polluted soil microcosms with Candida tropicalis diminishes phytoavailable chromium. J Appl Microbiol. 2013; 115(3):727-34. DOI: 10.1111/jam.12282. View

Mary Mangaiyarkarasi M, Vincent S, Janarthanan S, Rao T, Tata B . Bioreduction of Cr(VI) by alkaliphilic Bacillus subtilis and interaction of the membrane groups. Saudi J Biol Sci. 2013; 18(2):157-67. PMC: 3730869. DOI: 10.1016/j.sjbs.2010.12.003. View

Gu Y, Xu W, Liu Y, Zeng G, Huang J, Tan X . Mechanism of Cr(VI) reduction by Aspergillus niger: enzymatic characteristic, oxidative stress response, and reduction product. Environ Sci Pollut Res Int. 2014; 22(8):6271-9. DOI: 10.1007/s11356-014-3856-x. View

Shoaib A, Nisar Z, Nafisa , Javaid A, Khurshid S, Javed S . Necrotrophic fungus Macrophomina phaseolina tolerates chromium stress through regulating antioxidant enzymes and genes expression (MSN1 and MT). Environ Sci Pollut Res Int. 2019; 26(12):12446-12458. DOI: 10.1007/s11356-019-04457-y. View

Opperman D, Piater L, van Heerden E . A novel chromate reductase from Thermus scotoductus SA-01 related to old yellow enzyme. J Bacteriol. 2008; 190(8):3076-82. PMC: 2293266. DOI: 10.1128/JB.01766-07. View

Bae W, Lee H, Choe Y, Jahng D, Lee S, Kim S . Purification and characterization of NADPH-dependent Cr(VI) reductase from Escherichia coli ATCC 33456. J Microbiol. 2005; 43(1):21-7. View

Bopp L, Chakrabarty A, Ehrlich H . Chromate resistance plasmid in Pseudomonas fluorescens. J Bacteriol. 1983; 155(3):1105-9. PMC: 217804. DOI: 10.1128/jb.155.3.1105-1109.1983. View

Elangovan R, Abhipsa S, Rohit B, Ligy P, Chandraraj K . Reduction of Cr(VI) by a Bacillus sp. Biotechnol Lett. 2006; 28(4):247-52. DOI: 10.1007/s10529-005-5526-z. View

10.

Ye Q, Wu J, Wu P, Wang J, Niu W, Yang S . Enhancing peroxymonosulfate activation of Fe-Al layered double hydroxide by dissolved organic matter: Performance and mechanism. Water Res. 2020; 185:116246. DOI: 10.1016/j.watres.2020.116246. View

11.

Naumova E, Sukhotina N, Naumov G . Molecular-genetic differentiation of the dairy yeast Kluyveromyces lactis and its closest wild relatives. FEMS Yeast Res. 2004; 5(3):263-9. DOI: 10.1016/j.femsyr.2004.08.006. View

12.

Camargo F, Bento F, Okeke B, Frankenberger W . Hexavalent chromium reduction by an actinomycete, arthrobacter crystallopoietes ES 32. Biol Trace Elem Res. 2004; 97(2):183-94. DOI: 10.1385/BTER:97:2:183. View

13.

Barrera-Diaz C, Lugo-Lugo V, Bilyeu B . A review of chemical, electrochemical and biological methods for aqueous Cr(VI) reduction. J Hazard Mater. 2012; 223-224:1-12. DOI: 10.1016/j.jhazmat.2012.04.054. View

14.

Das S, Guha A . Biosorption of chromium by Termitomyces clypeatus. Colloids Surf B Biointerfaces. 2007; 60(1):46-54. DOI: 10.1016/j.colsurfb.2007.05.021. View

15.

Barak Y, Ackerley D, Dodge C, Banwari L, Alex C, Francis A . Analysis of novel soluble chromate and uranyl reductases and generation of an improved enzyme by directed evolution. Appl Environ Microbiol. 2006; 72(11):7074-82. PMC: 1636143. DOI: 10.1128/AEM.01334-06. View

16.

Aravindhan R, Madhan B, Rao J, Unni Nair B, Ramasami T . Bioaccumulation of chromium from tannery wastewater: an approach for chrome recovery and reuse. Environ Sci Technol. 2004; 38(1):300-6. DOI: 10.1021/es034427s. View

17.

Ruan B, Wu P, Chen M, Lai X, Chen L, Yu L . Immobilization of Sphingomonas sp. GY2B in polyvinyl alcohol-alginate-kaolin beads for efficient degradation of phenol against unfavorable environmental factors. Ecotoxicol Environ Saf. 2018; 162:103-111. DOI: 10.1016/j.ecoenv.2018.06.058. View

18.

Pal A, Dutta S, Paul A . Reduction of hexavalent chromium by cell-free extract of Bacillus sphaericus AND 303 isolated from serpentine soil. Curr Microbiol. 2005; 51(5):327-30. DOI: 10.1007/s00284-005-0048-4. View

19.

Srivastava S, Thakur I . Isolation and process parameter optimization of Aspergillus sp. for removal of chromium from tannery effluent. Bioresour Technol. 2005; 97(10):1167-73. DOI: 10.1016/j.biortech.2005.05.012. View

20.

Liu G, Yang H, Wang J, Jin R, Zhou J, Lv H . Enhanced chromate reduction by resting Escherichia coli cells in the presence of quinone redox mediators. Bioresour Technol. 2010; 101(21):8127-31. DOI: 10.1016/j.biortech.2010.06.050. View