Application of LC-MS/MS for Quantitative Analysis of Glucocorticoids and Stimulants in Biological Fluids

Overview

Journal J Pharm Anal

Specialty Chemistry

Date 2018 Feb 7

PMID 29403837

Citations 5

Authors

Jamshed Haneef

Mohammad Shaharyar

Asif Husain

Mohd Rashid

Ravinesh Mishra

Shama Parveen

Niyaz Ahmed

Manoj Pal

Deepak Kumar

Affiliations

Soon will be listed here.

Abstract

Liquid chromatography tandem mass chromatography (LC-MS/MS) is an important hyphenated technique for quantitative analysis of drugs in biological fluids. Because of high sensitivity and selectivity, LC-MS/MS has been used for pharmacokinetic studies, metabolites identification in the plasma and urine. This manuscript gives comprehensive analytical review, focusing on chromatographic separation approaches (column packing materials, column length and mobile phase) as well as different acquisition modes (SIM, MRM) for quantitative analysis of glucocorticoids and stimulants. This review is not meant to be exhaustive but rather to provide a general overview for detection and confirmation of target drugs using LC-MS/MS and thus useful in the doping analysis, toxicological studies as well as in pharmaceutical analysis.

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