Selective Elimination of Anti-DNA Antibody-producing Cells by Antiidiotypic Antibody Conjugated with Neocarzinostatin

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1986 Apr 1

PMID 2937806

Citations 7

Authors

T Sasaki

T Muryoi

O Takai

E Tamate

Y Ono

Y Koide

N Ishida

K Yoshinaga

Affiliations

Soon will be listed here.

Abstract

A new strategy was shown for the manipulation of autoantibody production in humans. Antiidiotypic antibody to human anti-DNA autoantibody was conjugated with neocarzinostatin (NCS), a cytotoxic agent, by using N-succimidyl 3-(2-pyridyldithio) propionate as a coupling agent. Human B cell clones, which produce anti-DNA autoantibodies, were killed by in vitro treatment with antiidiotype (Id)-NCS conjugates, while clones expressing an Id with irrelevant specificity were unaffected. These results indicate that treatment with anti-Id-NCS conjugates can act as a potent and specific means of generating immunosuppression of autoantibody production. This approach will have a significant advantage in aborting clones that are not effectively suppressed for the autoantibodies by anti-Id antibodies alone, and will result in a potential therapeutic treatment for systemic lupus erythematosus.

Citing Articles

Suppression of experimental systemic lupus erythematosus (SLE) with specific anti-idiotypic antibody-saporin conjugate.

Blank M, Manosroi J, Tomer Y, Manosroi A, Kopolovic J, Shoenfeld Y Clin Exp Immunol. 1994; 98(3):434-41.

PMID: 7994908 PMC: 1534502. DOI: 10.1111/j.1365-2249.1994.tb05509.x.

Anti-DNA antibodies. Their idiotypes and SLE.

Buskila D, Shoenfeld Y Clin Rev Allergy. 1994; 12(3):237-52.

PMID: 7804957 DOI: 10.1007/BF02802320.

Binding specificity of antiidiotypic autoantibodies to anti-DNA antibodies in humans.

Sasaki T, Muryoi T, Takai O, Tamate E, Saito H, Yoshinaga K J Clin Invest. 1988; 82(3):748-54.

PMID: 3262123 PMC: 303578. DOI: 10.1172/JCI113674.

Heterogeneity of anti-idiotypic antibodies to anti-DNA antibodies in humans.

Muryoi T, Sasaki T, HARATA N, Takai O, Tamate E, Yoshinaga K Clin Exp Immunol. 1988; 71(1):67-72.

PMID: 3258206 PMC: 1541633.

Incidence of anti-DNA idiotype-positive cells in human peripheral blood.

Takai O, Sasaki T, Muryoi T, HARATA N, Hashimoto H, Yoshinaga K J Clin Immunol. 1989; 9(4):313-21.

PMID: 2788658 DOI: 10.1007/BF00918663.

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