Systematic Analysis of MRNA and MiRNA Expression of 3D-Cultured Neural Stem Cells (NSCs) in Spaceflight

Overview

Journal Front Cell Neurosci

Specialty Cell Biology

Date 2018 Jan 30

PMID 29375320

Citations 6

Authors

Yi Cui

Jin Han

ZhiFeng Xiao

Yiduo Qi

Yannan Zhao

Bing Chen

Yongxiang Fang

Sumei Liu

Xianming Wu

Jianwu Dai

Affiliations

Soon will be listed here.

Abstract

Recently, with the development of the space program there are growing concerns about the influence of spaceflight on tissue engineering. The purpose of this study was thus to determine the variations of neural stem cells (NSCs) during spaceflight. RNA-Sequencing (RNA-Seq) based transcriptomic profiling of NSCs identified many differentially expressed mRNAs and miRNAs between space and earth groups. Subsequently, those genes with differential expression were subjected to bioinformatic evaluation using gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) and miRNA-mRNA network analyses. The results showed that NSCs maintain greater stemness ability during spaceflight although the growth rate of NSCs was slowed down. Furthermore, the results indicated that NSCs tended to differentiate into neuron in outer space conditions. Detailed genomic analyses of NSCs during spaceflight will help us to elucidate the molecular mechanisms behind their differentiation and proliferation when they are in outer space.

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