REST Regulates Distinct Transcriptional Networks in Embryonic and Neural Stem Cells

Overview

Journal PLoS Biol

Specialty Biology

Date 2008 Oct 31

PMID 18959480

Citations 105

Authors

Rory Johnson

Christina Hui-Leng Teh

Galih Kunarso

Kee Yew Wong

Gopalan Srinivasan

Megan L Cooper

Manuela Volta

Sarah Su-ling Chan

Leonard Lipovich

Steven M Pollard

R Krishna Murthy Karuturi

Chia-Lin Wei

Noel J Buckley

Lawrence W Stanton

Affiliations

Soon will be listed here.

Abstract

The maintenance of pluripotency and specification of cellular lineages during embryonic development are controlled by transcriptional regulatory networks, which coordinate specific sets of genes through both activation and repression. The transcriptional repressor RE1-silencing transcription factor (REST) plays important but distinct regulatory roles in embryonic (ESC) and neural (NSC) stem cells. We investigated how these distinct biological roles are effected at a genomic level. We present integrated, comparative genome- and transcriptome-wide analyses of transcriptional networks governed by REST in mouse ESC and NSC. The REST recruitment profile has dual components: a developmentally independent core that is common to ESC, NSC, and differentiated cells; and a large, ESC-specific set of target genes. In ESC, the REST regulatory network is highly integrated into that of pluripotency factors Oct4-Sox2-Nanog. We propose that an extensive, pluripotency-specific recruitment profile lends REST a key role in the maintenance of the ESC phenotype.

Citing Articles

REST Is Restless in Neuronal and Non-Neuronal Virus Infections: An In Silico Analysis-Based Perspective.

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PMID: 40006989 PMC: 11860772. DOI: 10.3390/v17020234.

Dysregulation of REST and its target genes impacts the fate of neural progenitor cells in down syndrome.

Huang T, Fakurazi S, Cheah P, Ling K Sci Rep. 2025; 15(1):2818.

PMID: 39843579 PMC: 11754635. DOI: 10.1038/s41598-025-87314-y.

Analysis of REST binding sites with canonical and non-canonical motifs in human cell lines.

Choi J, Lee E BMC Med Genomics. 2024; 17(Suppl 1):92.

PMID: 38632583 PMC: 11025195. DOI: 10.1186/s12920-024-01860-4.

REST Is Not Resting: REST/NRSF in Health and Disease.

Jin L, Liu Y, Wu Y, Huang Y, Zhang D Biomolecules. 2023; 13(10).

PMID: 37892159 PMC: 10605157. DOI: 10.3390/biom13101477.

Genome-wide Association Studies of REST Gene Associated Neurological Diseases/traits with Related Single Nucleotide Polymorphisms.

Wang J, Roy S, Richard S, Xu Y Curr Neurovasc Res. 2023; 20(3):410-422.

PMID: 37518996 PMC: 10556398. DOI: 10.2174/1567202620666230727153306.

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