Interaction Between the Centriolar Protein SAS-5 and Microtubules Facilitates Organelle Assembly

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2018 Jan 26

PMID 29367435

Citations 5

Authors

Sarah Bianchi

Kacper B Rogala

Nicola J Dynes

Manuel Hilbert

Sebastian A Leidel

Michel O Steinmetz

Pierre Gonczy

Ioannis Vakonakis

Affiliations

Soon will be listed here.

Abstract

Centrioles are microtubule-based organelles that organize the microtubule network and seed the formation of cilia and flagella. New centrioles assemble through a stepwise process dependent notably on the centriolar protein SAS-5 in SAS-5 and its functional homologues in other species form oligomers that bind the centriolar proteins SAS-6 and SAS-4, thereby forming an evolutionarily conserved structural core at the onset of organelle assembly. Here, we report a novel interaction of SAS-5 with microtubules. Microtubule binding requires SAS-5 oligomerization and a disordered protein segment that overlaps with the SAS-4 binding site. Combined in vitro and in vivo analysis of select mutants reveals that the SAS-5-microtubule interaction facilitates centriole assembly in embryos. Our findings lead us to propose that the interdependence of SAS-5 oligomerization and microtubule binding reflects an avidity mechanism, which also strengthens SAS-5 associations with other centriole components and, thus, promotes organelle assembly.

Citing Articles

Molecular architecture of the C. elegans centriole.

Woglar A, Pierron M, Schneider F, Jha K, Busso C, Gonczy P PLoS Biol. 2022; 20(9):e3001784.

PMID: 36107993 PMC: 9531800. DOI: 10.1371/journal.pbio.3001784.

PLK4 promotes centriole duplication by phosphorylating STIL to link the procentriole cartwheel to the microtubule wall.

Moyer T, Holland A Elife. 2019; 8.

PMID: 31115335 PMC: 6570480. DOI: 10.7554/eLife.46054.

A dynamically interacting flexible loop assists oligomerisation of the Caenorhabditis elegans centriolar protein SAS-6.

Busch J, Erat M, Blank I, Musgaard M, Biggin P, Vakonakis I Sci Rep. 2019; 9(1):3526.

PMID: 30837637 PMC: 6401066. DOI: 10.1038/s41598-019-40294-2.

Autoamplification and Competition Drive Symmetry Breaking: Initiation of Centriole Duplication by the PLK4-STIL Network.

Leda M, Holland A, Goryachev A iScience. 2018; 8:222-235.

PMID: 30340068 PMC: 6197440. DOI: 10.1016/j.isci.2018.10.003.

Revisiting Centrioles in Nematodes-Historic Findings and Current Topics.

Schwarz A, Sankaralingam P, OConnell K, Muller-Reichert T Cells. 2018; 7(8).

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