Stromalized Microreactor Supports Murine Hematopoietic Progenitor Enrichment

Overview

Journal Biomed Microdevices

Specialty Biomedical Engineering

Date 2018 Jan 22

PMID 29353324

Citations 1

Authors

Danika Khong

Matthew Li

Amy Singleton

Ling-Yee Chin

Biju Parekkadan

Affiliations

Soon will be listed here.

Abstract

There is an emerging need to process, expand, and even genetically engineer hematopoietic stem and progenitor cells (HSPCs) prior to administration for blood reconstitution therapy. A closed-system and automated solution for ex vivo HSC processing can improve adoption and standardize processing techniques. Here, we report a recirculating flow bioreactor where HSCs are stabilized and enriched for short-term processing by indirect fibroblast feeder coculture. Mouse 3 T3 fibroblasts were seeded on the extraluminal membrane surface of a hollow fiber micro-bioreactor and were found to support HSPC cell number compared to unsupported BMCs. CFSE analysis indicates that 3 T3-support was essential for the enhanced intrinsic cell cycling of HSPCs. This enhanced support was specific to the HSPC population with little to no effect seen with the Lineage and Lineage cells. Together, these data suggest that stromal-seeded hollow fiber micro-reactors represent a platform to screening various conditions that support the expansion and bioprocessing of HSPCs ex vivo.

Citing Articles

Co-culture of mesenchymal stem cell spheres with hematopoietic stem cells under hypoxia: a cost-effective method to maintain self-renewal and homing marker expression.

Amiri F, Kiani A, Bahadori M, Roudkenar M Mol Biol Rep. 2021; 49(2):931-941.

PMID: 34741711 DOI: 10.1007/s11033-021-06912-x.

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